Abstract: In order to study the number of microbes,especially Fusarium and Phytophthora,the greenhouse soil was sprayed with a series volumes of wood vinegar and then incubated at 25℃ for 35 days. The results showed that the pH of each treated soil changed little during the incubation.Generally,wood vinegar had no significant effect on soil pH except on the 7th days,except when the dose reached 3.3 mL/kg,the soil pH was significantly reduced by nearly 0.2 units.At initial period of incubation,the mineral nitrogen content in the soil sprayed with wood vinegar was slightly reduced for 15.1-28.2 mg/kg.However,as the incubation time prolonged,the content of mineral nitrogen in all treatments gradually increased to a similar level (324.9-344.8 mg/kg).The amounts of culturable bacteria,fungi and actinomycetes in the soils sprayed with different volumes of wood vinegar were significantly different from that in the control soil only on some days.But,on the 14th day, the number of culturable actinomycetes in the soil added with 1.3, 3.3 mL/kg wood vinegar was 31.4% and 34.4%,respectively,lower than that in the control soil.On the 21st day,the number of culturable bacteria in the soil added with 0.7,1.3 and 3.3 mL/kg wood vinegar was 20.2%,24.8% and 40.6% lower,respectively,lower than that in the control soil.And at this time,the number of culturable fungi in the soil added with 0.3,0.7 and 3.3 mL/kg was 45.9%,35.5%,36.6%,respectively,lower than that in the control soil.The amounts of Fusarium and Phytophthora were greatly changed in the soils added with different volumes of wood vinegar,however,they showed little difference from the control soil.In conclusion,wood vinegar,acting as an acidic liquid,had no significant effect on soil mineral nitrogen,culturable bacteria,fungi,actinomycetes,as well as the amounts of Fusarium and Phytophthora,even though there was a certain influence on soil pH.It could be speculated that wood vinegar had almost no impact on Fusarium and Phytophthora.Hence,it was not a suitable fumigant for soil,and its impact on alleviating soil-borne might be limited.

Key words: Wood vinegar, Soil, Fusarium, Phytophthora

CLC Number: 

• S472