cDNA Cloning and Expression Analysis of Cyclin-dependent Kinase (KdCDK) Gene in Kalanchoe daigremontiana

doi:10.7668/hbnxb.2017.03.006

Abstract

Abstract: To better understand the molecular mechanisms of CDKs involved in Kalanchoe daigremontiana,an A-Type CDK gene, KdCD K,was identified using rapid amplification of cDNA end(RACE)PCR. KdCDK gene consists of an ORF of 888 bp that was predicted to encode a 295 amino acid residue-long protein of 34.12 kDa with an isoelectric point of 6.15.KdCDK was related most closely to CmCDK and was clustered to CDKA subfamily.Real-time PCR analysis revealed that KdCDK transcript was expressed highly in root and upregulated under osmotic stress.This study characterized the novel KdCDK gene from Kalanchoe daigremontiana for the first time and the results would be useful for further functional determination of the gene.

Key words: Kalanchoe daigremontiana, Cyclin-dependent kinase, Gene expression

CLC Number:

ZHU Minqun, LIANG Xiaohong, HUANG Huiqing, OU Surong, GE Yongqiang, ZHANG Lijuan, ZHONG Tianxiu. cDNA Cloning and Expression Analysis of Cyclin-dependent Kinase (KdCDK) Gene in Kalanchoe daigremontiana[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(3): 33-41. doi: 10.7668/hbnxb.2017.03.006.

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