Abstract: In order to study the physiological mechanism of salicylic acid on alleviating aluminum toxicity of grape seedlings, solve the problem of aluminum toxicity in acid soil.Shuijing grape as material, using hydroponic method, in addition to the culture medium of 0.4 mmol/L aluminum chloride and different concentrations of salicylic acid were co-cultured for 5 weeks, after determination the indices of root, stem and leaf growth status, chlorophyll content, and active oxygen scavenging system and membrane lipid peroxidation of leaf and root.Effects of salicylic acid on the growth physiology of grape seedlings were analyzed under aluminum stress.The results show that under aluminum stress, plant growth was inhibited, leaf yellowing new leaves, root black.Chlorophyll content increased in old leaves and decreased in new leaves.SOD, POD and MDA contents increased in leaf and root and oxygen free radical production rate also increased, while root activity decreased.25, 50 μmol/L salicylic acid treatment significantly promoted the growth of grape seedling shoot stem, root color reddish, but 100 μmol/L salicylic acid treatment could inhibit the growth of grape seedlings, a small amount of root black.Salicylic acid treatment on the physiological effects of alleviate aluminum toxicity was obviously in grape seedlings.25, 50 μmol/L Salicylic acid treatment made chlorophyll and carotenoid contents were significantly higher than that of CK and single aluminum stress in old leaves, and the contents were significantly lower than single aluminum treatment.The SOD and POD activity in leaf and root were lower than single aluminum treatment, wheras the root POD activity was significantly higher.Production rate of oxygen free radical and malondialdehyde content treatment were lower than the single aluminum in leaf and root.In addition, 50 μmol/L salicylic acid treatment also significantly increased root vigor under aluminum stress.Thus, aluminum stress inhibition on the growth of grape plant significantly, showed strong poison, while salicylic acid treatment, had a certain effect on the remission of grape aluminum toxicity, among which 50 μmol/L salicylic acid treatment to the most obvious physiological effects alleviated aluminum toxicity in grape seedlings.

Key words: Salicylic acid, Grape, Aluminum stress, Physiological, Mechanism

CLC Number: 

• S663.01