Cloning and Identification of the B2A of Tianzhu White Yak KAP1.1 Sub Gene

doi:10.7668/hbnxb.2015.01.018

Abstract

Abstract: In this study, the high sulfur keratin B2A promoter of Tianzhu White Yak gene was cloned and got sequence, and analyzed the bioinformatics.Comparison of forecasting results by subcellular localization, phosphorylation analysis, secondary structure of proteins, tertiary structure, MEGA 5.10 software by NJ method and phylogenetic tree construction and MegAlign software to analyzed the difference between B2A gene and KAP1.1 gene. B2A gene CDS region was 471 bp, encoding a protein with 156 amino acids, the secondary structures were mainly composed of extended strand, beta turn and random coil.B2A and KAP1.1 protein of subcellular localization, phosphorylation analysis, conserved domains, secondary structure, tertiary structure and homology were different.But from the function prediction results showed that the two protein's function were exactly same, and Clustal X software was used to compare the amino acid sequence.B2A protein compared with KAP1.1 protein has ten amino acids deletion at position 33, the remaining sequence has very high conserved between two proteins.So it was proved that the B2A gene was KAP1.1 gene sub gene.

Key words: Yak, B2A gene, KAP1.1 protien, Sub gene

CLC Number:

Q78
S823.03

ZHANG Liang-bin, LIANG Chun-nian, PEI Jie, CHU Min, WU Xiao-yun, ZHANG Jian-yi, PAN He-ping, YAN Ping. Cloning and Identification of the B2A of Tianzhu White Yak KAP1.1 Sub Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 109-117. doi: 10.7668/hbnxb.2015.01.018.

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