Analysis of AcMNPV Meditated Expression of BmK IT and Vcath on the Apoptosis of Sf9 Cells

doi:10.7668/hbnxb.2015.01.019

Abstract

Abstract: In order to investigate the mechanism underlying the BmK IT mediated insecticidal activity of AcMNPV, an excitatory insect toxin, BmK IT, gene was inserted into the geneome of AcMNPV to construct a recombinant baculovirus.The scorpion insect neurotoxin BmK IT and vcath gene were constructed into the genome of AcMNPV using Bac-to-Bac system.Effects of BmK IT under these promoters of AcMNPV on Sf9 cells were analyzed and it revealed that the expression level of BmK IT was high under the regulation of PH promoter at 36, 48 h after the virus infected.Effects of AcMNPV mediated BmK IT and vcath synergism expression on the proliferation of Sf9 cells was also analyzed.Compared to AcMNPV-BmK IT (P10), the inhibition rate of AcMNPV-BmK IT (P10)-vcath(PH) increased 14.5% averagely, the expression level of c-Myc, cleaved-Caspase3 and Bax was raised and the expression of Bcl-2 was reduced.

Key words: AcMNPV, BmK IT, Vcath, Synergy

CLC Number:

ZHAO Jie, LIANG Ai-hua, FU Yue-jun. Analysis of AcMNPV Meditated Expression of BmK IT and Vcath on the Apoptosis of Sf9 Cells[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(1): 118-122. doi: 10.7668/hbnxb.2015.01.019.

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