SCAR Marker Resistance-related to Cucumber Alternaria Leaf Spot and Resistance Identification of Cucumber Germplasm

doi:10.7668/hbnxb.2014.01.037

Abstract

Abstract: In order to establish molecular marker-assisted selection system of cucumber alternaria leaf spot with F ₁,F ₂ and BC 1 population between a susceptible parent(L 63) and a resistant parent(L 9),a co-dominant AFLP marker was successfully converted into a simple,applied and co-dominant SCAR marker. Special marker SCEM 126 /122 were designed according to the sequence information of AFLP(E-CC / M-CAT) fragments. The marker was closely linked to the cucumber alternaria leaf spot resistance-related gene,and the genetic distance between the marker and the gene was 4. 4 cM. The marker could be useful in marker-assisted selection in cucumber breeding. The acquired SCAR marker had many advantages including fast,accurate,low cost,free from an environmental influence et. 64 resistant germplasm were abtained from total 290. Application of the acquired marker will inhance cucumber alternaria leaf spot disease resistant germplasm evaluation.

Key words: Cucumber, Alteruaria leaf spot, SCAR, Germplasm

CLC Number:

S642
2

WANG Hui-zhe, LI Shu-ju, YANG Rui-huan, GUAN Wei, DENG Qiang, CAO Ming-ming. SCAR Marker Resistance-related to Cucumber Alternaria Leaf Spot and Resistance Identification of Cucumber Germplasm[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(1): 208-212. doi: 10.7668/hbnxb.2014.01.037.

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