Diversity and Evolutionary Relationship of NBS-type Resistance Gene Analogues in Wheat

doi:10.7668/hbnxb.2011.04.003

Abstract

Abstract: Degenerate primers based on conserved motif(P-loop and GLPL)of the nucleotide binding site(NBS) region from the cloned plant disease resistance genes were used to isolate resistance gene analogues(RGAs) from genomic DNA of wheat.13 RGAs with uninterrupted open reading frames(ORFs) were obtained.Sequence identity among the 13 RGAs deduced amino acid sequences showed identity ranged from 36.9 % to 98.3%.The analysis of RGAs amino acid sequence structures suggested that they contained the domains such as P-loop,Kinase-2,Kinase-3a,and GLPL.The phylogenetic analyses for the deduced amino acids showed that RGAs from wheat were nonTIR type.These results showed that NBS type RGAs isolated from wheat might have the same origin and mechanism of evolution as that in other plants.

Key words: Wheat, NBS (Nucleotide binding site), RGA (Resistance gene analogues)

CLC Number:

ZHANG Li-rong, YANG Wen-xiang, LIU Da-qun. Diversity and Evolutionary Relationship of NBS-type Resistance Gene Analogues in Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(4): 23-26. doi: 10.7668/hbnxb.2011.04.003.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2011.04.003

http://www.hbnxb.net/EN/Y2011/V26/I4/23

References

[1] Knott D R. The wheat rust breeding for resistance[J].Theoretical and Applied Genetics,1989.191-198.
[2] 谭明谱. 水稻白叶枯抗性基因Xa22(t)的克隆[D].武汉:华中农业大学,2004.10-13.
[3] 高倩,王海燕,刘大群. 小麦抗叶锈病基因Lr35的RGA分析[J].华北农学报,2008,(06):50-53.
[4] 霍建飞,宋水山,李星. CaM及各亚型基因参与小麦抗叶锈病反应的研究[J].华北农学报,2010,(04):175-179.
[5] 杨静静,李星,李亚宁. 小G蛋白Rob2基因参与小麦抗叶锈病反应的研究[J].华北农学报,2010,(04):1-5.
[6] 李星,李亚宁,刘大群. 小麦抗叶锈病近等基因系TcLr41基因的差异表达[J].华北农学报,2008,(05):122-126.
[7] 刘松青,何莎,蒋芳. 小麦抗病基因同源序列(RGAs)的克隆与分析[J].中国农学通报,2007,(03):83-88.doi:10.3969/j.issn.1000-6850.2007.03.020.
[8] Feuillet C S,Travella N,Stein L. Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat(Triticum aestivum L.)genome[J].Proceedings of the National Academy of Sciences of the United States of America,2003,(25):15253-15258.doi:10.1073/pnas.2435133100.
[9] Leister D,Ballbora A,Salamin F. A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants[J].Nature Genetics,1996.421-429.doi:10.1038/ng1296-421.
[10] Collins N C,Webb C A,Seah S. The isolation and mapping of disease resistance gene analogs in maize[J].Mol Plant MicrobInteract,1998,(10):968-978.doi:10.1094/MPMI.1998.11.10.968.
[11] 黄萱,徐子勤,陈立余. 小麦NBS-LRR类抗病基因同源序列的分离与鉴定[J].分子细胞生物学报(英文版),2006,(02):91-96.
[12] Bozkurt O,Hakki E E,Akkaya M S. Isolation and sequence analysis of wheat NBS-LRR type disease resistance gene analogs using degenerate PCR primers[J].Biochemistry Genetics,2007,(5/6):469-486.doi:10.1007/s10528-007-9089-7.
[13] Monosi B,Wisser R J,Pennill L. Full-genome analysis of NBS-LRR-Encoding genes homologues in rice[J].Theoretical and Applied Genetics,2004,(7):1437-1447.doi:10.1007/s00122-004-1758-x.
[14] Palomino C,Satovic Z,Cubero J. Identification and characterization of NBS-LBR class resistance gene analogs in fababean(Vicia faba L.) and chickpea(Cicer arietinum L.)[J].The NRC Research Press,2006.1227-1237.
[15] Gill K S,Lubbers E L,Gill B S. A genetic linkage map of Triticum tauschii (DD) and its relationship to the D genome of bread wheat (AABBDD)[J].Genome/National Research Council Canada=genome/Conseil National De Recherches Canada,1991.362-374.
[16] Meyers B C,Dickerman A W,Michelmore R W. Plant disease resistance genes encode members of an ancient and diverse protein family with the nucleotidebinding superfamily[J].Plant Journal,1999,(3):317-332.doi:10.1046/j.1365-313X.1999.t01-1-00606.x.
[17] Yu J,Hu S N,Wang J. A draft sequence of the rice genome (Oryza sativa L.ssp.indica)[J].Science,2002,(5565):79-92.doi:10.1126/science.1068037.
[18] Mago R,Nari S,Mohan M. Resistance gene analogue from rice:cloning,sequencing and mapping[J].Theoretical and Applied Genetics,1999.50-57.doi:10.1007/s001220051207.
[19] Seah S,Sivasithamparam K,Karakousis A. Cloning and characterization of a family of disease resistance gene analogs from wheat and barley[J].Theoretical and Applied Genetics,1998,(5/6):937-945.doi:10.1007/s001220050974.

Please choose a citation manager

Content to export