Genetic Effects of PPARtr Gene on Carcass Traits in Anak×Gushi Chicken Resource Population

doi:10.7668/hbnxb.2010.06.003

Abstract

Abstract: A Anak×Gush resource population including 793 F₂ chickens was founded by F₂ design to analyze the PPARa gene polymorphism by the PCR-SSCP combined with sequencing techniques.The single nucleotide polymorphism(SNP)were found in exon 4 regions of the PPARa.Three kinds of genotypes detected in the resource population were defined as EE, EF and FF by digesting with BspH I restriction endonuclease.Effects of different geno.types on carcass traits were evaluated with SAS8.0 software.Results showed that the polymorphism of loci NC-006088：T32311C in PPARα gene was significantly related to the abdominal fat weight(AFW)and abdominal fat percentage(AFP)(P<0.05), and no significance with carcass weight, subcutaneous fat thickness, fat band width, semi—evisceration weight and evisceration weight(P>0.05).The chickens with the genotype of EE and EF had the higher AFW and AFP than those of FF(P<0.05).while there was no significant difference between the genotype of EE and EF(P>0.05).Result implied that PPARa gene affecting lipometabolism was a good marker for using in marker associated selection program.

Key words: hicken, F₂ Resource Population, PPARot gene, Polymorphism, Carcass trait

CLC Number:

S831

TIAN Ya-dong, KANG Juan-juan, SUN Gui-rong, HAN Rui-li, KANG Xiang-tao. Genetic Effects of PPARtr Gene on Carcass Traits in Anak×Gushi Chicken Resource Population[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 14-18. doi: 10.7668/hbnxb.2010.06.003.

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References

[1] 倪毓辉，郭锡熔.陈荣华.脂肪细胞分化的表型特征、分子标志和调控[]].国外医学儿科学分册，2004,31(6) :306一309.
[2] Issemann I,Green S. Activation of a member of the steroid hormone receptor super family by peroxisome proliferators [J]. Nature,1990,347:645一650.
[3] Schoonjans K,Peinado-Onsurbe A M,Heyman R A,et ad. PPARa. and PPAR}y activators direct tissue-specific transcriptional response via a PPRE in the lipoprotein lipase gene [J].The EMBO Journa1,1996,15:5336一5348.
[4] Kerenzvi S,Nir I ,Nit san Z. Effect of dietary concent rationas of fat and energy on fat deposition in broiler divergently selected for high or low abdominal adipose tissue[J].British Poultry Science,1992,33:517一524.
[5] Lemberger T ,Braissant 0,]uge-Aubry C,et al. PPAR tissue distribution and interactions with other hormone-signaling pathways[Jl.Annals of the New York Academy Sciences ,1996 ,804 ;231一251.
[6] Michalik L,Wahli W.Peroxisome proliferator-activated receptors:three isotypes for a multitude of functions[J].Current Opinion in Biotechnology,1999,10:564一570.
[7] Fajas L,Auboeuf D,Raspe E,et al. The organization,promotet analysis,and expression of the human PPAR gamma gene[J].Journal of Biological Chemistry, 1997, 272;18779一18789
[8] Schoon jans K,Watanabe M,Suzuki H,et aL.Induction of the acyl-coenzyme A synthetase gene by fibrates and fatty acids is mediated by a peroxisome praliferators response element in the C promoter[J].Journal of Biological Chemistry,1995 ,270 ;19269一19276.
[9] Fruchart J C > Brewer H B Jr, Leitersdorf E. Consensus for the use of fibrates in the treatment of dyslipoproteinemia and coronary heart disease. Fibrate Consensus Group[J]. American Journal of Cardiology,1998 ,181 ;912一917.
[10] 李晓轩，靳明明，田亚东，等.共扼亚油酸对固始鸡蛋品质和血清生化指标的影响[J].河南农业科学，2009(7);126一128.
[11] 靳明明;康相涛;孙桂荣，等.固始鸡与安卡鸡资源群体屠体性状的遗传分离特性[J].河南农业科学，2009(8);139一141.
[12] 杨宁.家禽生产学〔M].北京:中国农业出版社，2002:289一292.
[13] Kuniyasu A , Hayashi 5, Nakayama H. Adipocytes recognize and degrade oxidized low density lipoprotein through CD36 [ J ].Biochemical and Biophysical Research Communications , 2002 , 295 ; 319一323.
[14] Mossab A , Lessire M , Guillaumin S , Kouba M , Mourot J,Peiniau P, Hermier D. Effect of dietary fats on hepatic lipid metabolism in the growing turkey [ J ].Comparative Biochemistry and Physiology, Part B, Biochemistry&Molecular Biology,2002,132:473一483.
[15] 梅承君.固始鸡一安卡鸡F:代资源群血清生化指标与部分脂肪性状相关性及谷草转氨酶同工酶的研究[D].郑州:河南农业大学，2007.
[16] Everett L, Galli A, Grabb D. The role of hepatic peroxisome pmliferator-activated receptor(PPARs)in health and disease[J].Liver,2000,20:191一199.
[17] Vohl M C,Lepage P,Gaudet D,et al. Molecular scanning of the human PPARa gene; association of L162V mutation withmhyperapobetalipoproteinemia[J].Journal of Lipid Research,2000,41(6):945一952.
[18] Flavell D M,Pineda Toga I,Jamshidi Y, et al. Variation in the PPARa gene is associated with altered function in vitro and plasmlipid concentrations in type II diabetic nsubjects [ J ].Diabetologia,2000,43(5);673一680.
[19] Meng He, Wang Gui-Hua, Wang Qi-Gui,et ml. Studies of single nucleotide polymorphism of PPAR gene and its associations with fattiness trait in chicken仁J].Acta Genetica Sinica,2002 , 29 ( 2) ;119一123.
[20] 解相林，王栋，王慧，等. PPAft基因多态性与肉鸡脂肪性状的相关性研究【J].畜牧兽医学报，2005,36(12);1261一1264.

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