Variation of plgR Expression and Specific IgA Concentration in Mammary Gland of Mice after Immune Stimulus

doi:10.7668/hbnxb.2010.06.004

Abstract

Abstract: To study the effect of immune stimulus on plgR mRNA expression and specific IgA concentration in mammary gland.Forty eight KunMing white mice were divided into four groups, 12 mice per group.They were injected different vaccines as following at days 4 after parturition, A group(CK)isotonic Na chloride, B group Lipase+iso·tonic Na chloride, C group ISCOM, D group Lipase+LTB+ISCOM.The samples, including blood, milk and mammary gland issues, were collected at days 8 and days 12 after parturition.The specific IgA concentration in mouse plasma and milk was determined by indirect ELISA.The specific IgA concentration in mouse plasma was not significant among four groups(P<0.05), whereas that in moHse milk of B and D group at days 12 was higher than that at days 8.And the results of RealTime-PCR also demonstrated that the pIgR gene expression in mammary gland at days 12 ofgroup B and D was higher than that at days 8.Immune stimulus can enhance the expression of plgR mRNA expression and specific IgA concentration, and the pIgR gene over-expression could increase the specific IgA level in milk.

Key words: plgR, mRNA, IgA, Mammary gland

CLC Number:

S852.4

ZHAO Sheng-guo, WANG Jia-qi, ZHANG Chun-gang, BU Deng-pan, LIU Guang-lei, ZHANG Chun-lin, ZHANG Yu-tao, ZHOU Ling-yun. Variation of plgR Expression and Specific IgA Concentration in Mammary Gland of Mice after Immune Stimulus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(6): 19-24. doi: 10.7668/hbnxb.2010.06.004.

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