Microsatellite Analysis on Mutants of Peanut Induced by 60Co γ

doi:10.7668/hbnxb.2010.03.016

Abstract

Abstract: Mutagenic progenies M₂, selected from peanut variety Luhua11 irritated by γ ray of ⁶⁰Co,had significated difference on leaf characters and plant types.The DNA polymophisms of seven mutants were analysed by using 107 pairs of peanut SSR primers.The polymorphism was various among mutant materials and origional variety Luhua11.Mutant materials had more than two different loci from origional variety.Polymorphic analysis of SSR marker indicated that mutation can be induced by γ ray of ⁶⁰Co,mainly showing chromosome deficiency and chromosome duplication.The genetic similarity( GS) among 101 mutagenic progenies M₂ were calculated based on the data from SSR markers.The value of GS ranged from 0.457 to 1.000,with an average of 0.862.The variance ranges of genetic similarity of mutagenic progeny M₂ and their original variety Luhua11 were 0.692-1.000.The analysis of molecular markers indicated that abundant genetic variations can result and genetic bases in original varieties can be broadened from the γ-ray radiation．

Key words: Peanut( Arachis hypogaea L． ), SSR Marker, Irradiation inducement

CLC Number:

S565.2

CHEN Jing, HU Xiao-hui, MIAO Hua-rong, SHI Yun-qing, CUI Feng-gao, WU Lan-rong, SUN Ling-qiang, YU Shan-lin. Microsatellite Analysis on Mutants of Peanut Induced by ⁶⁰Co γ[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 68-72. doi: 10.7668/hbnxb.2010.03.016.

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