Optimization of SSR Amplification System and Agarose Gel Electrophoresis of Amplificons

doi:10.7668/hbnxb.2009.06.035

Abstract

Abstract: In this study,effect of five reaction components,including template DNA,dNTPs,primer,Mg⁺and Taq DNA polymerase,on the SSR amplification results were studied using wheat genomic DNA as PCR template.Mg+and Taq DNA polymerase showing most significant influence on PCR products,and then is template DNA,dNTPs have no significant effect on it.Furthermore,SSR markers on wheat chromosome 7B were screened by the optimized SSR system.Results indicated that,dominant SSR markers or codominant markers with significant difference on length can be separated by optimized SSR amplification system and high concentration agarose gel effectively.

Key words: Wheat, SSR, Molecular marker

CLC Number:

S512.03

LI Yong, NIU Yong-chun,. Optimization of SSR Amplification System and Agarose Gel Electrophoresis of Amplificons[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(6): 174-177. doi: 10.7668/hbnxb.2009.06.035.

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http://www.hbnxb.net/EN/Y2009/V24/I6/174

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