Abstract: Five invertases were investigated by semi2quantitative RT2PCR in different developing stages of the fruit, using genotype tomatoes,″ Momotaro″. The invertase genes expressions of tomato seedling under sub2high temperature,sub2 low temperature,NaCl salinity stress and osmotic stress were further studied. The results suggested that the expanding pe2 riod of fruit is affected by the expression of Lin5,Lin6 and Lin8.Only Lin8 expressed in mature green stage,while Lin6, Lin8 and AI2 expressed distinctly. The results showed that different members of acid invertase gene family regulated the different periods and tissues of tomato fruits development. Suboptimal temperature had significant influence on AI2. Whether sub2low temperature or sub2high temperature,both of them inhibited gene expression significantly,however,they have little effect on the expression of the cell wall2binding acid invertase genes (Lin5,Lin6 and Lin7) and have inhibited effect on Lin8. Lin5 and Lin7 have no expression in osmotic stress and salinity stress;however,the expressions of Lin6 and Lin8 are inhibited by osmotic stress and salinity stress. The expression was low when treated for three days,and for five days it was totally inhibited. AI2 is sensitive to osmotic stress whose expression is totally inhibited when treated for three days,meanwhile under prolonging the time of the salt stress,the expression is low,then completely disappeared.

Key words: Tomato, RT2PCR, Invertase, Gene expression

CLC Number: 

• Q786