ACTA AGRICULTURAE BOREALI-SINICA ›› 2009, Vol. 24 ›› Issue (3): 41-45. doi: 10.7668/hbnxb.2009.03.009

Special Issue: Biotechnology

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Construction of Constitutive and Cold-inducible Plant Expression Vector of CBF1 Gene

YANG Zhi ru1, YUN Jin feng1, WEI Jian hua2, XU Chun bo3   

  1. 1. Inner Mongolia Agricultural University,Huhhot 010019, China;

    2. Grassland Research Institute, Chinese Academyof Agriculture Sciences, Huhhot 010010, China;

    3. Beijing Agro 2biotechnology Research Center, Beijing 100097, China
  • Received:2009-03-20 Published:2009-06-28

Abstract: To improve the cold resistance in China alfalfa, gene of CBF1 transcription factor and its promoter were isolated from Arabido psis thaliana by PCR method. Twovectors for CBF1 expression in plant were constructed, one is in2 ducible which is driven by CBF1 promoter, another is constitutive which is driven by CaMV 35S promoter. This lay the foundation for improve the cold resistance in by transgenic transformation.

Key words: Arabido psis thaliana, CBF1, CBF1 promoter, Construction of expression vector

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Cite this article

YANG Zhi ru, YUN Jin feng, WEI Jian hua, XU Chun bo. Construction of Constitutive and Cold-inducible Plant Expression Vector of CBF1 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(3): 41-45. doi: 10.7668/hbnxb.2009.03.009.

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