Hypersensitive Response and Activity Dynamics of Defense Enzymes Induced by Elicitor(s) from Wheat-stem Rust Interaction System

doi:10.7668/hbnxb.2009.01.018

Abstract

Abstract: Elicitors can be found in intercellular washing fluids(IWFs) in highly incompatible interaction betweenwheat and stem rust. The IWFs were classified using Ammonium Sulfate Precipitation and all the proteins dissolved inddH₂0₂ were injected into highly incompatible interaction wheat leaves respectively,then hypersensitive response was ob-served and activity dynamics of peroxidase(POD) and phenylalanine ammonia-lyase(PAL) were measured. The resultshowed that elicitor (s) mainly was (were) included in the 50%-80%classification and obviously induced the hypersensi-five response of wheat leaves. POD and PAL activity had remarkable increase after inducement by 40μg/ mL protein solu-tion of 50%-80%classification. POD activity reached highest point at the time of 36 h and had 37. 47%increase,andthat of PAL activity were 60 h and 78. 20%.

Key words: Wheat, Elicitor, Hypersensitive response, Peroxidase, Phenylalanine ammonia-lyase

CLC Number:

S435.121

HAN Jian-dong, CAO Yuan-yin, YAO Ping. Hypersensitive Response and Activity Dynamics of Defense Enzymes Induced by Elicitor(s) from Wheat-stem Rust Interaction System[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 79-82. doi: 10.7668/hbnxb.2009.01.018.

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References

1 Holden D W，Roland Rohringer. Proteins in intercellular washing fluid from nonioculated and rust-ai3ected leaves of wheat and barley [J].Plant Physiology，1985, 78:715- 723.
2 Itogel K,Beissmann B, Reisener H J.ecific binding of a hypersensitive Lignification elicitor from Puccinia grcuninis. f. sp.Tritfcf to the plasma membranes from wheat [J].Plan- to,1988,183：164一169.
3 Sutherland M W,Deverall B J.Wheat cultivar and chrormso- coals selectivity of two types of eliciting preparations from rust pathogens [J].Physiological and Molecular Plant Pathology， 1989,35：535一541.
4 Sutherland M W,Deverall B J. The ubiquity of con-specific eliciting activity in intercellular washing fluids from rust-in- fected wheat leaves [J].Plant Patla}logy,1990,39：50一57.
5 刘占良.小资叶锈菌互作的胞问洗脱液对防卫反应的诱发效应[D]保定:河北农业大学，1995.
6 土庆梅，土智忻，刘娟，等.感染叶锈菌的小麦细胞问隙液中激发子的定性及初步分离[J]植物生理学报， 2000,26 (5)：427一432.
7 徐建强.我国小麦和小麦秆锈菌互作的组织学、细胞学及互作产物研究[D]沈阳:沈阳农业大学，2004.
8 马云鹏，陈琐，韩胜芳，等.感染叶锈菌的小麦细胞问隙液对小麦悬浮细胞程序性死亡的诱导[J]河北农业大学学报，2005,28 (3)：12一16.
9 Ebel J,Cosio E G. Elicitors of plant defense response [J].lnt Rev(乌201,1994,148：1一36.
10 曾永三，王振中.虹豆与锈菌互作中的多酚氧化酶和过氧化物酶活性及其与抗性的关系[J]植物保护学报，2004,31(2)：145一149.
11 王智忻，杨秀屏，刘娟，等.小麦叶锈菌侵染对小同抗病性小麦品种的生理生化差异[M]//赵可夫.植物抗性生理研究.济南:山东科技出版社，1991： 173 - 178.
13 贾显禄，王振中，王平.水稻与稻瘟病菌非亲和性互作中重要防御酶活性变化规律研究[J].植物病理学报，2002,32 (3)：206一213.
13 李云锋，土振中，贾显禄.稻瘟菌细胞壁激发子活性物质的提取与性质[J].华中农业大学学报，2004,23 (3)285一289.
14 邵敏.稻白叶枯病菌外泌蛋白激发子的活性测定及其纯化[J].中国生物防治，2004,20 (4)：260一263.
15 Tlburzy R,Reisener HJ. Resistance of wheat to Pucciniagramfnfs f.sp. triticf：association of the hypersensitive reac-lion with the cellular accumulation of lignin-like materialand callow [J].Physiol Mol Plant Pathol,1990,36:109-120.
16 Andrea P，Tilman O，Friederike S. Apoplastic peroxidasesand lingir"ication in needles of Norway spruce [J].PlantPhysiol,1994,106:53一60.
17邓恩新，王文忠，王智听一种简易的向植物薄叶片内注射溶液的装置[J]植物生理学通讯，1992,28 (4): 296.
18 贾显禄.小麦与小麦秆锈菌互作及非亲和机制研究 [D].沈阳:沈阳农业大学，1997.
19 Schai3rath U, Scheinpllug H, Reisendr H J.An elicitor from pyricularia argzae induces resistance responses on rice:iso- lation，characterization and pHYBiological properties [J]. Physiol Mol Patlal,1995,46：293一307.
20 林植芳，李双顺，张东林，等.采后荔枝果皮色素、总酚及有关酶活性的变化[J].植物学报，1988,30(1)：40- 45.
21 Baker C J.Recongition responses in pathogen/ nonlast and race/ cultivar interactions involving soybean and Pseu- 由rmnas syringae pathovars [J].Physiol Mol Plant Pathol， 1993,43：81一94.
22 李振歧，商鸿生.中国农作物抗病性及其利用[M].北京:中国农业出版社，2005.
23 李宏潮，土虹，胡道芬.植物低聚糖提取和生物活性鉴定[J].华北农学报，1992,7(1)：60一64.
24 Stafford H A. "Ihe metabolism of aromatic compounds[J]. Ann Rev Plant Physiol,1974,25：459一486.
25 李云锋.稻瘟菌激发子CSBI诱导水稻防御性相关酶的活性变化[J]作物学报，2004,30 (6)：613一617.

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