Study on Genetic Diversity and Relationship in Watermelon Germplasms by AFLP Analysis

doi:10.7668/hbnxb.2007.S1.043

Abstract

Abstract: 57 watermelon materials were analyzed using AFLP.The results showed that 709 AFLP bands were ampli??fied using 18 primer pairs, among them, 440( 61. 4%) bands were polymorphic. Based on DNA bands, the similarity coefficient were calculated and their average value was 0. 70, ranging from 0. 24 to 099,which indicated that relationship wasclose and their genetic base was narrow among materials of watermelons.When all of the polymorphic bands were analyzedby cluster analysis, 57 watermelon materials were classified into 2 groups. Group included 1 wild watermelon material, andgroup, 56 watermelon materials fell into it. Among the group introduced watermelon materials were clustered together aswell as the F1 and parent materials. In addition, this study demonstrates that AFLP fingerprint ing has higher resolution andcan reveal the difference of cult ivars, so it is an efficient tool.

Key words: Watermelon, Cultivar, Genetic diversity, Relationship, AFLP analysis

CLC Number:

S651

LIYan-mei, DUANHui-jun, MAZhi-ying. Study on Genetic Diversity and Relationship in Watermelon Germplasms by AFLP Analysis[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2007.S1.043.

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