Study on Preparation Methods of Exserohilum turcicum for Intact Chromosome DNA

doi:10.7668/hbnxb.2007.06.036

Abstract

Abstract: Several preparation methods for Exserolalum tureicum intact chromosome DNA were comparatively stuied, in order to establish an all-sided system to analysis the molecular karyotype of this fungi. The results showed that chromosome DNA of Exserohalum tungzaghubum were only obtained by the agarose-embedding protoplasts method, other methods gave incompetent results. Moreover, two chromosomal bands were separated by PFGE, both were more than 2200 kb.

Key words: Exserohilum turcicum, Intact Chromosome DNA, Electrophoretic karyotype

CLC Number:

S432.1

LIU Zhen-pan, WANG Yan-hui, DONG Jin-gao. Study on Preparation Methods of Exserohilum turcicum for Intact Chromosome DNA[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(6): 176-178. doi: 10.7668/hbnxb.2007.06.036.

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http://www.hbnxb.net/EN/Y2007/V22/I6/176

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