Expression of K+ Channel Gene MIRK in Muskmelon

doi:10.7668/hbnxb.2007.04.011

Abstract

Abstract: A 1330 bp cDNA fragment of K⁺ channel gene MIRK (Melon Inward Rectifying K⁺ Channel) was isolated from leaves of muskmelon (Cucumis melo var. reticulatus Naud.). The nucleotide sequence and its deduced amino acid sequence analysis displayed that the cDNA fragment was located in the 5'-terminal of MIRK gene and contained six transmembrane segments S1-S6 and a GYGD (Gly-Tyr-Gly-Asp) motif. Phylogenetic analysis showed that MIRK was belonged to the KAT1 sub-family and most close to the SIRK of Vitis vinifera. The results of semi-quantitive RT-PCR displayed that MIRK was preferentially expressed in leaves and fruits, and also expressed in female flowers and shoots, but nearly not expressed in roots, indicating that MIRK might play a key role in the muskmelon development.

Key words: Muskmelon (Cucumis melo var. reticulatus Naud.), K⁺ channel gene, MIRK, Sequence analysis, Expression pattern

CLC Number:

S652.03

ZHANG Yi-dong, HUANG Dan-feng, NIU Qing-liang, ZUO Kai-jing. Expression of K⁺ Channel Gene MIRK in Muskmelon[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 46-51. doi: 10.7668/hbnxb.2007.04.011.

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