Construction and Expression of the Eukaryotic Expression Plasmid of Porcine Immunoglobulin G Fc Receptor Ⅱ

doi:10.7668/hbnxb.2007.04.012

Abstract

Abstract: The Fc receptor cDNA sequence of porcine IgG II cloned by us was used to design primers. Using RT-PCR technology, a 960 bp of swFcγRII sequence covering the whole coding region was amplified from the peripheral blood leucocyte cDNA, and the was sub-cloned into the expression vector pcDNA3. Through double digestion by Kpn I/EcoR I and PCR identification, a 5375 bp and a 901 bp of DNA fragments were obtained, indicating that the recombinant plasmid pcDNA/swFcγRII was successfully constructed. The 901 bp fragment was then transfected into COS-7 cells by the lipofection method, and the ligand affinity of swFcγRII was identified by rosetting procedures.

Key words: Pig, Fc-RII, Express edvector, Rosetting

CLC Number:

S828.03

ZHANG Yu-yang, ZHANG Gai-ping, QIAO Song-lin, GUO Cheng-liu. Construction and Expression of the Eukaryotic Expression Plasmid of Porcine Immunoglobulin G Fc Receptor Ⅱ[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 52-55. doi: 10.7668/hbnxb.2007.04.012.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.2007.04.012

http://www.hbnxb.net/EN/Y2007/V22/I4/52

[1]	WANG Ya, WANG Yuetao, SHEN Guanwang, WANG Fuhua, WANG Shengxuan, BAI Tao, YIN Haiqing. Improvement of Rice Blast Resistance by Pyramiding the R Gene Pigm and the Non-R Gene bsr-d1 [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 157-165.
[2]	SHI Yumei, CHEN Shaokang, XING Kai, ZHAO Yanhui, YUAN Jiani, SHENG Xihui, QI Xiaolong, NI Hemin, GUO Yong, WANG Chuduan. Identification of Differential Genes of Longissimus Dorsi Muscle of Songliao Black Pig and Landrace Pig Based on High-throughput Sequencing Technology [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(5): 222-229.
[3]	FU Yanfeng, ZHAO Weimin, LI Hui, LI Bixia, CHENG Jinhua, XU Xiaobo, REN Shouwen. Genome Wide Association Analysis of Litter Size in Pigs [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(4): 232-238.
[4]	ZHANG Jingjing, LI Bing, SHI Yufan, GAO Xiurui, PAN Xiuqing, SONG Xue, WU Yanrong. Transcriptome Sequencing and Gene Expression Analysis of Watermelon Peel with Different Firmness [J]. Acta Agriculturae Boreali-Sinica, 2022, 37(3): 44-52.
[5]	LONG Xi, ZHAO Jiugang, CHAI Jie, PAN Hongmei, ZHANG Liang, ZHANG Tinghuan. Screening of Innate Immune Candidate Genes in Rongchang Pigs and Landrace [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(S1): 373-380.
[6]	WANG Liang, LI Zhongqiu, TANG Xiaodong, ZHANG Haifeng, LIU Di, ZHANG Dongjie. *Screening and Analysis of the Core Region of Porcine ATP6V0A4* Gene Promoter** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2021, 36(3): 216-221.
[7]	LONG Xi, ZHANG Tinghuan, GUO Zongyi, CHAI Jie. *Cloning and Transcriptional Activity Analysis of Porcine STAB2* Gene Promoter** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 346-351.
[8]	CHEN Ying, LIU Bin, ZENG Yangshuang, LI Qiang, LIAO Kun, TAN Ya, ZHANG Shunhua, ZHU Li. Comprehensive Evaluation of Meat Quality in Qingyu Pig at Different Ages [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(S1): 352-357.
[9]	SONG Xue, WANG Shuyan, CHEN Yuanyuan, WANG Pei, HUO Hailong, ZHANG Xia, HUO Jinlong. *Cloning, Expression and Functional Bioinformatics Analysis of B4GALNT2* Gene Related to Immune Rejection in Banna Mini-pig Inbred Line** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(6): 219-226.
[10]	WANG Xiaolong, LIU Fengzhi, SHI Xiangbin, WANG Xiaodi, JI Xiaohao, WANG Zhiqiang, WANG Baoliang, ZHENG Xiaocui, WANG Haibo. Effects of Organic Fertilizers on Root Growth and Soil Nutrition of Grape [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(5): 177-184.
[11]	RU Shuhua, LÜ Dongying, XU Wanqiang, LIU Xiao, HOU Limin, SUN Shiyou, ZHANG Guoyin, WANG Ling. Effects of Continuous Application of Chicken and Pig Waste Compost on Soil and Wheat Copper and Zinc Accumulation [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(S1): 215-221.
[12]	WANG Yanping, ZHU Rongsheng, WANG Huaizhong, WANG Cheng, HU Hongmei. Selection of Appropriate Reference Genes for RT-qPCR Analysis in Different Tissue with Different Development Periods in Dapulian Pigs [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(S1): 45-53.
[13]	CHEN Ying, LIU Bin, LI Qiang, SHUAI Surong, TAN Ya, ZHANG Shunhua, ZHU Li. Effects of Closed-flock Reproduction on Carcass Performance, Meat Quality of Tibetan Pigs of Different Generations [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(S1): 59-63.
[14]	LONG Xi, CHAI Jie, ZHAO Jiugang, LAN Jing, GUO Zongyi, ZHANG Tinghuan. *Cloning,Transcriptional Activity and Regulatory Elements Analysis of Porcine EIF2S3* Gene Promoter** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(S1): 38-44.
[15]	LI Rui, XU Haixia, WU Wei, WANG Suying, REN Mengke, ZHANG Pengpeng, XU Yongjie. *Molecular Cloning, Temporal and Spatial Expression Patterns, and Subcellular Localization of Porcine TAK1* Gene** [J]. ACTA AGRICULTURAE BOREALI-SINICA, 2018, 33(5): 68-75.

Please choose a citation manager

Content to export