Abstract:
Young leaves, petioles and calli of Pinellia ternata Breit were transformed with GUS gene via Agrobacterium tumefaciens. The effects of different explants, infection period, concentration of Agrobacterium tumefaciens, days of co-culture and pre-culture, and strains on the transient expression of GUS gene were analyzed. The result indicated that a higher transient express rate of GUS gene could be acquired from the explant petioles and calli which were dipped into Agrobaterium suspension EHA102 or EHA105 (OD600 adjusted to 0.2) for 15 min, and co-cultured for 3 days and 2 days, respectively, in darkness.
Key words:
Pinellia ternata Breit,
Agrobacterium tumefaciens,
GUS gene,
Transient express
CLC Number:
JIA Yong-fang, MA Yu-kun, GUO Yu-long, LI Ming-yang. Research on the Transient Express of GUS Gene by Agrobacterium tumefaciens in Pinellia ternata Breit[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(4): 42-45. doi: 10.7668/hbnxb.2007.04.010.