Expression of BoLA- DRB3 mRNA in Chinese Holstein Cows

doi:10.3969/j.issn.1000-7091.2013.04.004

Abstract

Abstract: To study the regulation of upstream regulatory region polymorphisms on BoLA- DRB3 gene expres- sion，PCR- SSCP and DNA sequencing were used to identify the linkage relationship between BoLA- DRB3* exon2 and BoLA- DRB3 URR．Combined with the predicted results of binding mode between different URR and transcrip- tion factor，three linkage types were selected to detect the BoLA-DRB3 mRNA levels by relative Real- time PCR ex- periments． The results showed that the expression abundances of BoLA-DRB3 varied in different linkage types.The expression level of mRNA of BoLA- DRB3 gene linked with H17H17 was significant higher than H1H1 and H5H6 (P＜0.01)．This preliminary proved that the polymorphism of upstream regulatory region can cause the expression change of BoLA- DRB3 structural gene．

Key words: Cow, BoLA- DRB3 gene, Polymorphism of URR, Real- time PCR

CLC Number:

WANG Xue-qing, LI Kui-ying, PEI Cui-juan, ZHANG Feng, WU Zhan-jun, LIU Xiao-hu, MA Shu-lin, ZHANG Xin-tong, WANG Kun. Expression of BoLA- DRB3 mRNA in Chinese Holstein Cows[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 19-22. doi: 10.3969/j.issn.1000-7091.2013.04.004.

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