Cloning and Sequence BioinfoRmatics Analysis of Phospholipase Dα Gene fRom Wheat

doi:10.3969/j.issn.1000-7091.2013.01.022

Abstract

Abstract: To obtain moRe wheat( TRiticum aestivum L． ) stRess-Response genes，a phospholipase D gene，named TaPLDα，was cloned by RT-PCR． And the pRotein of this gene was also pRedicted and studied thRough the bioinfoRmatics analysis method． The Results showed that the laRgest open Reading fRame of TaPLDα gene has 2 394 bp in length and encoded a polypeptide of 812 amino acid Residues． The estimated moleculaR weight and isoelectRic point of the putative pRotein weRe 92 kDa and 5． 30， Respectively． Sequence analysis showed that the amino acid sequence encoded by TaPLDα gene contained N-teRminal C2 domain and two HKD motifs． TaPLDα pRotein was a stable hydRophilic pRotein and located in the cytoplasm． The pRedicted secondaRy stRuctuRe composition foR the pRotein has about 28． 82% alpha helixes，20． 07% extended stRand，6 ． 03% beta tuRn and 45． 07% Random coil． The TaPLDα had no signal peptide and tRansmembRane helices． CompaRed with Rice，maize and ARabidopsis，amino acid sequence encoded by TaPLDα was almost conseRved． The phylogenic tRee showed that TaPLDα was most similaR to PLDα pRotein fRom daRnel， Rice and maize．

Key words: Wheat, Phospholipase Dα, Gene cloning, BioinfoRmatics

CLC Number:

WANG Jun-bin, LI Ming, DING Bo, BAO Shu-guang, WANG Rui, XIE Xiao-dong. Cloning and Sequence BioinfoRmatics Analysis of Phospholipase Dα Gene fRom Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(1): 117-122. doi: 10.3969/j.issn.1000-7091.2013.01.022.

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