Study on Transferring LFY Gene into Gala Via Agrobacterium tumefaciens Mediation

doi:10.3321/j.issn:1000-7091.2007.02.014

Abstract

Abstract: The factors affecting apple transformation mediated by Agrobacterium tumefaciens, such as co-cultivation time, bacterium density et al., had been studied through GUS transient expression. Apple of Gala was transformed by Agrobacterium tumefaciens EHA105 carrying LFY gene from Arabidopsis. It was proved that the target LFY gene had been integrated into the genome of regenerated plants by PCR analysis.

Key words: Agrobacterium tumefaciens-mediated, Genetic transformation, LFY, GUS, Apple

CLC Number:

S661.1

LIU Jing, LI Xiang-li, XU Ji-zhong, SHAO Jian-zhu. Study on Transferring LFY Gene into Gala Via Agrobacterium tumefaciens Mediation[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 53-55. doi: 10.3321/j.issn:1000-7091.2007.02.014.

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