Studies on Improving the Frequency of Isolated Microspore-derived Embryos in Brassica napus L.

doi:10.3321/j.issn:1000-7091.2007.01.027

Abstract

Abstract: The application of microspore culture technique was restricted because of its low frequency of embryogenesis. Two methods of enhancing the frequency of embryogenesis were employed in the study, namely, activated charcoal and 6-BA treatment in NLN-13 media. Eleven Brassica napus genotypes (TR1-11) were used for isolated microspore culture. Among the 11 genotypes, 10 produced microspore-derived embryos. The production of microspore-derived embryos varied remarkably among these varieties. Embryo yield per bud is from 0.08 to 3.53. TR4 and TR9 were the best genotypes for microspore culture and their frequency of embryogenesis is 3.23 and 3.53, respectively. When treated with 0.5 mg/L activated charcoal after adding 0.2 mg/L 6-BA in NLN-13 media, TR4 and TR9 produced 7.11 and 10.05 embryos per bud respectively, 2.88 and 6.52 higher than CK. Average embryo yield increased after pretreated in 33℃ for 24 hours. Microspore-derived embryos on B5 medium with 6-BA 0.2 mg/L and 3% Sucrose and 1% agar was good for regenerated plants. When microspore embryoid developed into the globular stage and early torpedo stage, adding fresh medium and transferring the cultures under shaking would greatly improve the synchronization of embryo development and reduce the abnormal embryoids.

Key words: Brassica napus L., Isolated microspore culture, Frequency, Microspore-derived embryos

CLC Number:

S634.301

TIAN Bao-ming, JIANG Wu-sheng, ZHANG Xiao-wei, YUAN Yu-xiang, GENG Jian-feng, CAO Gang-qiang, HAN Yong-ping, ZHAO Zhen, LIAN Yu-li. Studies on Improving the Frequency of Isolated Microspore-derived Embryos in Brassica napus L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(1): 116-119. doi: 10.3321/j.issn:1000-7091.2007.01.027.

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