Abstract: The influence factors to microspore embryogenesis of non-heading Chinese cabbage were studied through seventeen genotypes. The result showed that the best time for sampling was 7 to 14 days after the first bud flowering on the main anthotaxy. The capacity of embryoid production varied significantly in different genotypes. Among them, twelve genotypes yield embryos successfully, which the rate could reach 71%. Genotype 102 was an ideal material for embryoid induction, and the frequency could reach 32.78 embryos per bud. Genotype that with a highest yield produced 546 times as many embryos as that with a lowest yield. The embryoid induction ability of normal bolting genotypes were higher than easy bolting and hard bolting genotypes. The embryoid induction frequency increased when microspores were cultured in medium that supplemented with B₅ organic compound instead of NLN organic compound, especially for genotypes that difficult in producing embryos. Mannitol did not contribute to microspore division.

Key words: Non-heading Chinese cabbage, Isolated microspore culture, Embryoid

CLC Number: 

• S634.01