Several Factors Affecting the Vacuum Infiltration Gene Transformation in Non-heading Chinese Cabbage

doi:10.3321/j.issn:1000-7091.2003.04.010

Abstract

Abstract: Several factors affecting the vacuum infiltration transformation efficiency in Chinese cabbage (Brassica parachinensis Bailey) were studied.The developmental status of seedlings,vacuum infiltration time,type of Agrobacterium tumefaciens used,all influenced the transformation frequency.Via selection by herbicide Basta in the progeny and identification of the occurrence of the target gene by PCR, the transformation frequency was compared in different treatments.It was concluded that using the nopaline type of Agrobacterium,5 min vacuum infiltration,and using the plants with just several blooming flowers would give the best results.

Key words: Vacuum infiltration transformation, Insect-resistant genes, Non-heading Chinese cabbage

CLC Number:

CAO Chuan-zeng, LIU Fan, ZHAO Hong, CAI Min-hua, XU Heng-jian. Several Factors Affecting the Vacuum Infiltration Gene Transformation in Non-heading Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(4): 35-38. doi: 10.3321/j.issn:1000-7091.2003.04.010.

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