Analysis of Population Structure of Non-heading Chinese Cabbage Based on SSR Marker

doi:10.3969/j.issn.1000-7091.2013.03.012

Abstract

Abstract: To study population structure within non-heading Chinese cabbage crops，78 non-heading Chinese cabbage varieties grown in a large planting area in China were collected． Their DNA polymorphisms were analyzed by a set of SSR markers and their population structure were clustered by Structure 2． 1 software according SSR data． Out of SSR primer 120 pairs， 21 pairs origining 10 linkage groups of Brassica rapa were screened out with polymorphism and 56 alleles in the 78 varieties were generated． The average number of alleles per SSR locus was 2． 7． The 56 markers were imported in the program structure to identify subpopulations， resulting in 4 subgroups in the 78 varieties．Subpopulations S1 and S2 mainly included 49 Pak choi varieties from different provinces in southern or northern China． Seedling Chinese cabbages were mainly distributed in subpopulation S3 and S4． Wutacai varieties were mainly distributed in subpopulation S1 and S4． The genetic backgrouds of Caixin varieties were relatively complicated． The relationship between material grouping and geographical origin was very clear in subpopulation S2． This result indicated an obvious population structure within non-heading Chinese cabbage，which established the basis for utilizing the current germplasm resources to guide breeding and discovering the new genes．

Key words: Non-heading Chinese cabbage, SSR, Population structure, DNA polymorphism

CLC Number:

S634.03

Qin Yanmei, Li Xiang, Zhao Jianjun, Wang Yanhua, Xuan Shuxin. Analysis of Population Structure of Non-heading Chinese Cabbage Based on SSR Marker[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 62-66. doi: 10.3969/j.issn.1000-7091.2013.03.012.

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http://www.hbnxb.net/EN/Y2013/V28/I3/62

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