The Optimization of SRAP Amplification Program and System in Non-heading Chinese Cabbage

doi:10.7668/hbnxb.2010.04.026

Abstract

Abstract: Using flowering Chinese cabbage (Brassica parachinensis L. H. Bariley) genome DNA as template，the major components of SRAP including concentrations of Mg²⁺，dNTPs，Taq DNA polymerase，primers and template DNA were optimized in this study by orthogonal design with five factors and four levels respectively. The optimum SRAP-PCR reaction system suit for non-heading Chinese cabbage was established. Using this system，five accessions of non-heading Chinese cabbages were used as template DNA to be amplified by 30 SRAP primer pairs designed from Brassica genus. Seven SRAP primer pairs revealed two bands or more in 5 non-heading Chinese cabbage cultivars were selected. The result shows the optimization of SRAP System in non-heading Chinese Cabbage in stable.

Key words: Non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino), SRAP(Sequence-related amplified polymorphism), PCR, Orthogonal design

CLC Number:

S63.1

LI Xiang, XUAN Shu-xin, WANG Yan-hua,ZHAO Jian-jun, SHEN Shu-xing. The Optimization of SRAP Amplification Program and System in Non-heading Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(4): 119-122. doi: 10.7668/hbnxb.2010.04.026.

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