A Study on Improving Frequency of Isolated Microspore-derived Embryos of Chinese Cabbages

doi:10.3321/j.issn:1000-7091.2005.06.010

Abstract

Abstract: Studies on factors for enhancing theembryogenic rates in isolated microspore culture of chinese cabbages were carried out.The results showed that 21 varieties had produced microspore-derived embryos among 24 varieties of chinese cabbage cultured in NLN-13 liquid medium.The production of microspore-derived embryos varied remarkably among these varieties.Embryo yield per bud is from 0.1 to 14.66.Sucrose and hormone concentrations in NLN medium were compared.NLN with 13% sucrose is better in production of microspore-derived embryos.Average embryo yield is higher in 33℃ high temperature treatment from 24 to 48 hour.The B₅ medium with 6-BA 0.2mg/L,3%Sucrose and 1% agar is good for microspore-derived embryo to regenerated plants.

Key words: Chinese cabbage, Isolated microspore culture, Frequency, Microsporederived embryos

CLC Number:

S634.1

JIANG Wu-sheng, YUAN Yu-xiang, ZHANG Xiao-weiGENG Jian-feng, HAN Yong-ping, GAO Mu-qiang, LI Gen-yi. A Study on Improving Frequency of Isolated Microspore-derived Embryos of Chinese Cabbages[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2005, 20(6): 34-37. doi: 10.3321/j.issn:1000-7091.2005.06.010.

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