Development of EST -SSR Markers in Peanut (Arachis hypogaea L .)

doi:10.7668/hbnxb.2009.S1.011

Abstract

Abstract: 53 177 ESTs from Genbank database in NCBI and 4 074 ESTs from Unigene library of E12 (Arachis hypogaea L.)in our laboratory are analysed.Totally 11 260 non -redundant ESTs contigs are detected.1 323 SSR loci are searched by software analysis in NCBI database and Unigene library ,mainly di -and tri -nucleotide repeat motif except of few tetra -nucleotide repeat motif and compound repeat.In these EST -SSR loci,average length is 18.88 bp and average 8.5 ESTs contain one SSR loci.The AG/TC and CTT/GAA motif are most frequent types,accounting for 39.3 % and 22.6 % in dinucleotide and trinucletide repeats,respectively.

Key words: Peanut, EST, SSR, Frequency, Characteristics

CLC Number:

S565.03

WANGJin-yan, PANLi-juan, YANGQing-li, YUShan-lin. Development of EST -SSR Markers in Peanut (Arachis hypogaea L .)[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.011.

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