ACTA AGRICULTURAE BOREALI-SINICA ›› 2006, Vol. 21 ›› Issue (4): 123-130. doi: 10.3321/j.issn:1000-7091.2006.04.031

Special Issue: Aquatic products

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Exogenous Xylanase Influences the Activities and Distribution of Six Digestive Enzymes in Gaster, Intestine and Hepatopancreas of Tilapia nilotica L.

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  1. 1. College of Life Sciences, Henan Normal University, Xinxiang, 453007, China;
    2. Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, 453003, China;
    3. College of Aqua_life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China
  • Received:2006-03-02 Published:2006-08-28

Abstract: Wheat basal diet was control.The tested diets were wheat basal diet added with different level of xylanase(0.05%,0.10%,and 0.15%).Each treatment was devised with 5 repeats and each repeat had 40 male Tilapia nilotica L.All the fishes lived in floating cages were fed 4 times a day and each time were fed to satiation.75 days later the activities of proteinase,amylase,lipase,xylanase,cellulose and pectinase were determined.The results indicated that:The activities of proteinase and cellulose in gaster of the 0.10% experimental group increased significantly compared with those of the control(P<0.01).The activities of the six digestive enzymes in intestine of all experimental groups increased in different levels.Especially,the activities of all the six digestive enzymes in intestine of the 0.10% experimental group increased significantly(P<0.01).Exogenous xylanase inhibited the activities of proteinase,amylase and lipase in hepatopancreas,but promoted the activities of endogenic xylanase,cellulose and pectinase.Adding exogenous xylanase did not effect on the distribution of amylase,endogenic xylanase,cellulose and pectinase in gaster,intestine and hepatopancreas,but had distinct influences on the distribution of proteinase and lipase.

Key words: Xylanase, Tilapia nilotica L., Digestive enzyme, Exogenous enzyme, Endogenic enzyme

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