Abstract: The fermentation process of high yield xylanase strain(Bacillus subtilis)BE-91 was optimized by One-Factor-at-a-Time Experimentation Method and Uniform Design Method. The optimal fermentation process were as follows: trypton (made in China) 1.5 %, yeast extract (made in China) 0.05%, beef powder 0.3%, KH₂PO₄ 0.2%, MgSO₄ 0.03%, NaCl 0.5%, tween 80 0.4%, initial pH 9.2, shake flask capacity 150 mL/500 mL, inoculure concentration 3.5%, stirring rate 180 r/min, temperature 37℃. Under the optimal fermentation process, xylanase activity was up to 423.24 U/mL after 8 h of cultivation, 1.5 times in comparison with the original fermentation process. So the strain BE-91 had advantages of short fermentation period and high thermostable acid xylanase activity in China.

Key words: Uniform design method, Xylanase, Xylanase activity, Fermentation process

CLC Number: 

• TQ925