Abstract: Cotton aphid Aphis gossypii (Homoptera: Aphidae) is one of the seriously occurred non??target pests in transgenicBt cotton fields. The activit ies and specific activit ies of midgut digestive enzymes, including proteinase, amy?? lase, trehalase and sucrase, of cotton aphids fed on Bt cotton cv. GK12 for different generat ions were detected with microtiter plate reader, and were compared with those of cotton aphids fed on the non??Bt parental cotton cv. simian 3. Thus the short??term and long??term effects of Bt cotton on the digest ive enzymes of cotton aphids were determined. The re?? sults showed that there were no significant differences in the activities and specific activities of the four digest ive enzymes between the cotton aphids fed on Bt cotton and non??Bt parental cotton, no matter for one to three generations, or for over sixty generations. And there were no significant difference among the cotton aphids fed on Bt cotton for different generations. It indicated that Bt cotton had no significant impacts on the activities of cotton aphid??s midgut digestive enzymes both in the short run and long term.

Key words: Transgenic Bt insect??resistant cotton, Cotton aphid, Digestive enzyme

CLC Number: 

• S435.62