Polymorphism Comparison of AFLP and SSR Between Scab Resistant Cucumber Materials

doi:10.3321/j.issn:1000-7091.2006.03.024

Abstract

Abstract: AFLP and SSR analysis were performed among scab resistant parents,F₂ plants and their bulked extremes.Their rates of polymorphism and positive marker linked to target gene was compared.The results indicated that the rates of polymorphism of AFLP,SSR were 36.5% and 22% respectively;the positive rates were 9.6% and 0.AFLP primer pair E20M64 was found closely linked to target gene,genetic distance was 4.83cM.SSR primer CSWCT02B was linked to target gene with genetic distance of 28.7cM.Polymorphism and positive marker rates of AFLP was higher than that of SSR.The advantages and disadvantages,and application of two kinds of molecular marker system in selection of linkage markers to target gene and gene tagging were also discussed.

Key words: Cucumber, Scab, Molecular marker, AFLP, SSR

CLC Number:

S46.421.1+9

SUN Xiao-hong, DU Sheng-li, ZHANG Gui-hua, WANG Bao-li, GUO Ai-guang, PEI Guo-liang. Polymorphism Comparison of AFLP and SSR Between Scab Resistant Cucumber Materials[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 105-107. doi: 10.3321/j.issn:1000-7091.2006.03.024.

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