Construction of Plant Transformation Vector of PpDREB2 Gene

doi:10.3321/j.issn:1000-7091.2006.02.005

Abstract

Abstract: The plant transformation vector p3301-BI121-PpDREB2 was constructed in this experiment.The Pp-DREB2 gene was cloned into pGEX-KG vector firstly,and then was ligated to p3301-BI121 vector.The gene was controlled by CaMV35S promoter and the selective marker gene was PPT(phosphinothricin).Then the recombinant plasmid was transferred into Agrobacterium Tumefaciens EHA105 by direct entering way.

Key words: Poa pratensis, PpDREB2 gene, Plant transformation vector

CLC Number:

REN Qing, LIU Guang-jie, GUO Xiu-lin, SHEN Shi-hua. Construction of Plant Transformation Vector of PpDREB2 Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(2): 18-22. doi: 10.3321/j.issn:1000-7091.2006.02.005.

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