Study on Diversity of Wheat Salt-Tolerance LCT Gene

doi:10.3321/j.issn:1000-7091.2006.02.002

Abstract

Abstract: LCT1 is probably one of important way of absorbing Na⁺ and Cs⁺ in bread wheat (Triticum aestivum L.).Thereafter,Mining LCT1 natural diversity in wheat is important to improve salt-tolerance by traditional breeding and genetics engineering.PCR primers corresponding to LCTl cDNA sequence(NCBI Accession:AF015523) was used to the study on LCT natural diversity in eight wheat accessions.Based on LCT sequences blast,10 kinds of LCT sequences were found.They were named LCT2-1,LCT 2-2,LCT2-3,LCT3-1,LCT3-2,LCT3-3,LCT3-4,LCT3-5,LCT3-6 and LCT3-7.The result showed that 10 kinds of LCT sequences are all different from LCT1.Deletion mutations in LCT2-1,LCT2-3,LCT3-2,LCT3-3,LCT3-4,LCT3-6 and LCT3-7 were all happened on LCT N-terminal.Point mutants in 10 kinds of LCT sequences all led to amino-acid change.The LCT kinds were different in different wheat accessions.Four LCT kinds were found in Chadianhong and only one in Bainong 3217 and Sumai 3.LCT3-1 exists in six wheat accessions.

Key words: Wheat, Salt-tolerance, LCT(low-affinity cation transporter), Diversity

CLC Number:

S512.01

LI Meng-jun, ZHOU Rong-hua, JIA Ji-zeng. Study on Diversity of Wheat Salt-Tolerance LCT Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(2): 6-9. doi: 10.3321/j.issn:1000-7091.2006.02.002.

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