Production of Agrobacterium-mediated Transgenic Potato Plants Containing phaG Gene

doi:10.3321/j.issn:1000-7091.2006.01.001

Abstract

Abstract: phaG is a key enzyme in PHA_S biosynthesis process.In order to produce medium-chain-length polyhydroxyalkanoates(mcl-PHA_S)in transgenic plants,plant expression vector pTiBHS-2 containing phaG cloned from Burkholderia caryophylli(BcphaG) was constructed.A major commercial cultivar of potato,Atlantic,was transformed via Agrobacterium tumefaciens mediated procedure.Nine transgenic plants are confirmed positive by PCR analysis.Western blot analysis demonstrated that BcphaG gene is expressed.The effect of over-expressing BcphaG gene on plant growth and development were also analyzed.At the early stage,compared to control plant,the growth of over-expressing lines is inhibited,root regeneration is delayed.However,these transgenic plants recover gradually after roots regenerated.It suggested that plant growth and development is partially affected by over-expressing BcphaG gene.

Key words: Potato, Medium-chain-length polyhydroxyalkanoates, phaG gene

CLC Number:

S632.01

SUN Huan-xing, ZHANG Xiu-hai, WU Zhong-yi, HUANG Cong-lin, LI Wen-bin. Production of Agrobacterium-mediated Transgenic Potato Plants Containing phaG Gene[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(1): 1-4. doi: 10.3321/j.issn:1000-7091.2006.01.001.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.3321/j.issn:1000-7091.2006.01.001

http://www.hbnxb.net/EN/Y2006/V21/I1/1

References

[1] Snell K D,Peoples O P.Polyhydroxyalkanoate polymers and their production in transgenic plants[J].Matabolic Engineering,2002,4:29-40.
[2] Mittendorf V,Robertson E J,Leech R M,et al.Synthesis of medium-chain-length polyhydroxyalkanoates in Arabidopsis thaliana using intermediates of peroxisomal fatty acid β-oxidation[C].Proceeding of the National Academy of Sciences of the United States America,1998,95:13397-13402,
[3] Poirier Y,Dennis D E,Klomparens K,et al.Polyhydroxyalkanoates,a biodegradable thermoplastic,produced in transgenic plants[J].Science,1992,256:520-523.
[4] Valentin H E,broyles D L,Casagrande L A,et al.PHA production,from bacteria to plants [J].International Journal of Biological Macromolecules,1999,25:303-306.
[5] Lossl A,Eibl C,Harloff H J,et al.Polyester synthesis in transplastomic tobacco (Nicotiana Tabacum L.):significant contents of polyhydroxybutyrate are associated with growth reduction[J].Plant Cell Report,2003,21:891-899.
[6] Houmiel K L,Slater S,broyles D,et al.Poly(β-hydroxybutyrate) production in oilseed leucoplasts of Brassica napus[J].Planta,1999,209:547-550.
[7] Poirier Y.Production of polyesters in transgenic plants [J].Advances in Biochemical Engineering Biotechnology,2001,71:209-240.
[8] 叶梁,宋艳茹.生物技术生产生物可降解塑料PHB的研究进展[J].生物技术通报,1998,3:9-16.
[9] brumbley S M,Purnell M P,Petrasovits L A,et al.Development of sugarcane as a biofactory for biopolymers.International Symposium on Biological Polyesters Program & Abstracts[M].Beijing:Tsinghua University,2004.54-55.
[10] 雍伟东,梁铁兵,谢安勇,等.PhbB,phbC基因在大肠杆菌中的表达及对马铃薯植株的转化和检测[J].植物学报,1998,40:615-621.
[11] Nawrath C,Poirier Y,Somerville C.Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation[C].Proceeding of the National Academy of Sciences of the United States America,1994,91:12760-12764.
[12] Poirier Y,Ventre G,Caldelari D.Increased flow of fatty acids toward β-oxidation in developing seeds of Arabidopsis deficient in diacylglcerol acyltransferase activity or synthesizing medium-chain-length fatty acids [J].Plant Physiology,1999,121:1359-1366.
[13] Moire L,Rezzonico E,Goepfert S,et al.Impact of unusual fatty acid synthesis on futile cycling through beta-oxidation and on gene expression in transgenic plants[J].Plant Physiology,2004,134:432-42.
[14] Gross R A,Kalra B.Biodegradable polymers for the environment[J],Science,2002,297:803-807.
[15] Bohmert K,Balbo I,Kopka J,et al.Transgenic arabidopsis plants can accumulate polyhydroxybutyrate to up to 4%of their fresh weight[J].Planta,2000,211:841-845.
[16] Sambrook J,Russell D.分子克隆实验指南(第三版)[M].北京:科学出版社,2002.
[17] 张鹤龄,李天然,哈斯阿古拉,等.抗卷叶病毒(PLRV)转基因马铃薯栽培种及其抗病性研究[J].病毒学报,1995,342-350.
[18] 王关林,方宏筠.植物基因工程(第二版)[M].北京:科学出版社,2002.
[19] 叶梁,王涛,宋艳茹.转基因植物生产生物可降解塑料的研究进展[J].科学通报,1999,1249-1257.

Please choose a citation manager

Content to export