Betaine Accumulation and Aldehyde Betaine Dehydrogenase Enzyme Activity for Transgenic Wheat under Stress Condition

doi:10.3321/j.issn:1000-7091.2003.z1.009

Abstract

Abstract: Assay for salt tolerance and drought resistance of t ransgenic wheat with BADH cDNA by biolist icmethod were conducted under simulated salt/drought stress condition. The results indicated that the t ransgenicwheat has many obvious advantages over its received plant s,such as the more vigorous development of seedlings,the well developed root system and the greater root act ivity under salt/ drought stress condition,as well as theimproved plasma membrane protection of ex cised leaf. The betaine aldehyde dehydrogenase enzyme activity underdif ferent salt concentration is also hig her,and more betaine accumulation under drought condition,than it sreceived plant. This indicated that the int roduction of BADH gene into wheat may affect a series of physiolog icalreaction to adapt st ress condition,this may be one of the reasons for its higher salt/drought tolerance. The w ayto increase crop stress tolerance/ resistance through transgenic method may be effect ive.

Key words: Transgenic, Transgenic w heat, BADH, Drought resistance, Salt tolerance

CLC Number:

S512.01

ZHANG Yan-min, DING Zhan-sheng, WEN Zhi-yu, JIANG Chun-zhi, LI Hui, HUO Yun-qian, ZHU Zhi-qing, CHEN Shou-yi, GUO Bei-hai. Betaine Accumulation and Aldehyde Betaine Dehydrogenase Enzyme Activity for Transgenic Wheat under Stress Condition[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3321/j.issn:1000-7091.2003.z1.009.

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