Effects of VB1 and Desiccation on Callus Culture of Wheat Immature Embryos

doi:10.3321/j.issn:1000-7091.2003.04.006

Abstract

Abstract: The effects of VB_(1) and desiccation on callus induction and bud differentiation of immature embryo from 14 wheat genotypes were studied.Result indicated:there was a significant difference between the various genotypes in bud differentiation frequency,and ZhengXin 992 was the highest(71.9%).VB_(1)(10 mg/L) supplemented into the induction?subculture and differentiation media can improved the embryogenic callus development and increased the bud differentiation frequency.12 h desiccation before differentiation culture improved the bud differentiation frequency of calli.

Key words: Wheat, Genotypes, VB_(1), Desiccation, Callus induction and differentiation

CLC Number:

S512.035.3

GUO Xiang-yun, YIN Jun, YU Gui-rong, SONG Li. Effects of VB1 and Desiccation on Callus Culture of Wheat Immature Embryos[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(4): 19-22. doi: 10.3321/j.issn:1000-7091.2003.04.006.

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