Acta Agriculturae Boreali-Sinica

ACTA AGRICULTURAE BOREALI-SINICA ›› 2003, Vol. 18 ›› Issue (1): 44-46. doi: 10.3321/j.issn:1000-7091.2003.01.013

Special Issue: Cucumber； Biotechnology；

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The Obtaining of Transgenic Cucumbers Resistant to Herbicide and Resistance Detection of T1 Plants

JIN Hong1, DU Sheng li2, CHEN Zheng1, WEI Ai min2   

  1. 1 Central Lab of Tianjin Academy of Agricultural Sciences, Tianjin 300192, China;
    2 Tianjin Cucumber Research Institute, Tianjin 300192, China
  • Received:2002-10-23 Published:2003-03-28

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Abstract: The procedure of cucumber trans formation was established by s tudying factors such as types of explants,cotransformation with Agrobacterium tume fac iens and adding acetosyringone or not etc. The antiherbicide gene bar was transferred into the c otyledons of the cucumber father M1 by Agrobacterium tume faciens and the t rans formed cucumber lines were obtained by taking through the proces s of cal lus di fferentiation, shooting and rooting.The PCR detection showed 13 lines showed the existence of the bar gene fragment. T1 plants were got by selfcrossing of T0 plants, 84% of which showed higher resis tance to 1 000 dilution of the herbicide of BASTA.

Key words: Cucumber, Bar gene, Transformation, Resistance characterization

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JIN Hong, DU Sheng li, CHEN Zheng, WEI Ai min. The Obtaining of Transgenic Cucumbers Resistant to Herbicide and Resistance Detection of T1 Plants[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(1): 44-46. doi: 10.3321/j.issn:1000-7091.2003.01.013.

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