A Comparative Study on Peroxidase Isozymes of K-type, V-type Male Sterile Line and Maintenance Line in Common Wheat

doi:10.3321/j.issn:1000-7091.1997.02.002

Abstract

Abstract: By means of vertical slab polyacrylamide gel electrophoresis,the isozyme zymograms of peroxidase were analyzed in the stamen,pistil and flag leaf from plants of Ae.kotschyi cytoplasmic male sterile line (K-CMS),and of Ae.ventricosa cytoplasamic male sterile line (V-CMS) as well as its maintenance line (B) at different developmental stages. The results showed that the isozyme zymograms of anthers of K-CMS,V-CMS and B were different at various developmental stages. According to the change of the isozyme bands and activity,it was found that the stage of pollen abortion was different between K-CMS and V-CMS. It was supposed that the key stage of pollen abortion was "single nucleus stage"for V-CMS, but "double nucleus stage"for K-CMS,This was in accordance with the observation of cytology.The isozyme zymograms of pistil and flag leaf of K-CMS,V-CMS,B showed no significantly different at different stages.

Key words: Common wheat, Peroxidase isozyme, K-type male sterile line, V-type male sterile line, Maintenance line

CLC Number:

S512.035

Ju Zhengchun, Sun Lanzhen. A Comparative Study on Peroxidase Isozymes of K-type, V-type Male Sterile Line and Maintenance Line in Common Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1997, 12(2): 7-11. doi: 10.3321/j.issn:1000-7091.1997.02.002.

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