Abstract: Two cultivars (Ho Mei,Tokyotakenoko)were used and the influence of growth conditions of donor plants on microspore culture in Chinese cabbage was investigated.The embryo yield of "Ho Mei" and the proportion of torpedo and cotyledonary embryos were much higher than those of "Tokyotakenoko". When the donor plants of "Ho Mei" were cultivated under long day length conditions (14, 16, 18hr),the embryo yields, the number of embryos which developed shoots longer than 2 cm and the percentage of plantlert regeneration were much higher than those cultivated under the short day length (12hr).But there were no significant differences between the donor plants cultivated under 14, 16, 18hr conditions. In the temperature condition study,the highest embryo yield, was obtained from the plants cultivated under the 20℃ growth condition. The results of "Tokyotakenoko" were different from those of "Ho Mei". When the donor plants of "Tokyotakenoko"cultivated under the 14hr condition, the embryo yield, the number of embryos which developed shoots longer than 2 cm and the percentage of plantlet regeneration were highest.With the donor plants under the short day length condition (12hr ),very few embryos were found and almost no plantlet was formed. The highest embryo yield was obtained from the plants under 15℃ and 14hr day length growth conditions.

Key words: Chinese cabbage, Microspore culture, Environmental condition

CLC Number: 

• S634.301