A Study on the Esterase Isoenzyme of the Variants Derived from Barley DNA Introduction into Common Wheat

doi:10.3321/j.issn:1000-7091.1994.01.008

Abstract

Abstract: Five stable plant-lines from resistance variants in D2 generation derived from introducing DNA of two-rowed barley (H. vulgare ssp. distichon Hsu) with resistance to powdery mildew into susceptable common wheat `Hua 76'via the pollen-tube pathway were analysed with esterase isoenzyme (EST). The results of EST detection showed that the lines increased one band which was present in the donor but was absent in the receptor, named as transgene enzyme band. Thus it is confirmed that barley genes (or DNA segments) were integrated into or recombined with receptor's wheat genome and expressed. The effects of exogenous DNA introduced into recipient on variation of the character and change of the isoenzyme activity in the progeny were also discussed.

Key words: Wheat, Exogenous DNA introduction, Powdery mildew resistance, Esterase isoenzyme

CLC Number:

S512.101

Yan Xinfu, Liu Wenxuan, Lin Qiuping. A Study on the Esterase Isoenzyme of the Variants Derived from Barley DNA Introduction into Common Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1994, 9(1): 39-43. doi: 10.3321/j.issn:1000-7091.1994.01.008.

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