High Frequency Cell Division and Callus Formation in the Culture of Protoplast Derived from Embryogenic Callus of Wheat

doi:10.3321/j.issn:1000-7091.1994.01.001

Abstract

Abstract: The compact and granular calli were selected from anther-induced calli of wheat varieties CK89, 102,113 and immature inflorescence-induced calli of Huadong, respectively.While these calli were cultured in the modified MS medium with 2-4mg/L 2,4-D,a friable fast-growing type of embryogenic calli was obtained after 6 to 10 weeks. The high yield protoplasts were isolated from the culture and the protolast yield was 1 to 3.7 × 10'/g calli.The isolated protoplasts were cultuerd in PCMZ medium solidified with 0.4% agarose. The first division occured 3-5 days after plating .The second and third divisions were identifiable during 7 to 14 days. A large number of cell aggregates emerged after two weeks culture, and the division frequency was 20.3%-73.3%. After 4-6 weeks plating, the microcalli formed.The calli were obtained from wheat varieties CK89, 102 and Huadong, and cell aggregates were obtained from 113. An experiment on calli differentiation is under way. A few factors offecting the protoplast cultures were also studied.

Key words: Wheat, Embryogenic calli, Protoplast culture, Protoplast-derived calli

CLC Number:

S512.101

Cheng Zengshu, Gao Zhiqiang, Fang Ren. High Frequency Cell Division and Callus Formation in the Culture of Protoplast Derived from Embryogenic Callus of Wheat[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1994, 9(1): 1-6. doi: 10.3321/j.issn:1000-7091.1994.01.001.

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