Genetic Diversity Analysis and Primary Core Germplasm Development of Upland Cotton Germplasm Resources

doi:10.7668/hbnxb.20194138

Abstract

Abstract:

The aim of this study was to evaluate the genetic diversity of upland cotton germplasm resources and screen representative germplasm,so as to provide theoretical basis for scientific evaluation and efficient utilization of upland cotton germplasm resources.Based on the SNP markers of 367 upland cotton resources and the phenotypic data of 353 resources,genetic diversities were analyzed,phylogenetic tree and primary core germplasm were constructed,and effects of primary core germplasm construction were evaluated.The results showed that the polymorphism information content(PIC)of the original upland cotton population was 0.24,and the genetic diversity index of each phenotypic trait was close to 2.00,which were similar to the results of previous studies.The upland cotton germplasm could be divided into three groups based on SNP markers,and 73 genotypic primary core resources were constructed.The Nei's genetic diversity index,PIC and other genetic diversity indexes of primary core germplasm were higher than those of original germplasm.Based on the phenotypic data,the upland cotton germplasm could be divided into three groups,and the mean value of each trait showed the trend that the group Ⅰ was the smallest,the group Ⅱ was in the middle,and the group Ⅲ was the largest.Seventy phenotypic primary core resources were constructed.Compared with the original germplasm,the mean values of all traits were not significantly different,the range and genetic diversity index were similar to that of the original germplasm,and the coefficient of variation was higher than that of the original germplasm.Fifteen primary core resources constructed based on SNP markers overlapped with those constructed based on phenotypic data.Most of these resources were genotypic group Ⅱ and phenotypic group Ⅱ.These results showed that the genotypic genetic diversity of upland cotton germplasm resources was low,but the phenotypic variation was rich and the phenotypic genetic diversity was high.The genotypic and phenotypic primary core germplasm constructed in this study had a good representation of genetic diversity and could be used as a typical germplasm for preservation and utilization.

Key words: Upland cotton, Genetic diversity, Core germplasm, SNP marker, Phenotypic traits

ZHANG Xi, LIU Yi, QIAN Yuyuan, WANG Guang'en, WANG Hanju, JIAO Xiufen, XIE Hui, CUI Shufang, LI Junlan. Genetic Diversity Analysis and Primary Core Germplasm Development of Upland Cotton Germplasm Resources[J]. Acta Agriculturae Boreali-Sinica, 2023, 38(S1): 26-33. doi: 10.7668/hbnxb.20194138.

/ / Recommend / Download Citations

URL: http://www.hbnxb.net/EN/10.7668/hbnxb.20194138

http://www.hbnxb.net/EN/Y2023/V38/IS1/26

Figures/Tables 9

References 22

[1]	袁有禄, 魏晓文, 毛树春, 潘境涛, 方红曼, 吕慧颖, 邓向东, 葛毅强, 魏珣, 杨维才. 棉花育种行业创新与进展[J]. 植物遗传资源学报, 2018, 19(3):455-463.doi:10.13430/j.cnki.jpgr.2018.03.010.
	Yuan Y L, Wei X W, Mao S C, Pan J T, Fang H M, Lü H Y, Deng X D, Ge Y Q, Wei X, Yang W C. Genetic and breeding progress of cotton[J]. Journal of Plant Genetic Resources, 2018, 19(3):455-463.
[2]	孔繁玲, 姜保功, 张群远, 杨付新, 李如忠, 刘永平, 赵素兰, 郭腾龙. 建国以来我国黄淮棉区棉花品种的遗传改良Ⅰ.产量及产量组分的改良[J]. 作物学报, 2000, 26(2):148-156.doi:10.3321/j.issn:0496-3490.2000.02.004.
	Kong F L, Jiang B G, Zhang Q Y, Yang F X, Li R Z, Liu Y P, Zhao S L, Guo T L. Genetic improvements of cotton varieties in Huang-Huai region in China since 1950s I.improvements on yield and yield components[J]. Acta Agronomica Sinica, 2000, 26(2):148-156.
[3]	郑怀国, 赵静娟, 秦晓婧, 贾倩, 齐世杰. 全球作物种业发展概况及对我国种业发展的战略思考[J]. 中国工程科学, 2021, 23(4):45-55.doi:10.15302/J-SSCAE-2021.04.022.
	Zheng H G, Zhao J J, Qin X J, Jia Q, Qi S J. Overview of the global crop seed industry and strategic thinking on its development in China[J]. Strategic Study of CAE, 2021, 23(4):45-55. doi: 10.15302/J-SSCAE-2021.04.022 URL
[4]	代攀虹, 孙君灵, 贾银华, 杜雄明, 王谧. 利用表型数据构建陆地棉核心种质[J]. 植物遗传资源学报, 2016, 17(6):961-968.doi:10.13430/j.cnki.jpgr.2016.06.001.
	Dai P H, Sun J L, Jia Y H, Du X M, Wang M. Construction of core collection of upland cotton based on phenotypic data[J]. Journal of Plant Genetic Resources, 2016, 17(6):961-968. doi: 10.13430/j.cnki.jpgr.2016.06.001
[5]	Frankel O H. Genetic Perspectives of Germplasm Conservation[M]//Arber W K,Llimensee K,Peacock W J,Stralinger P,eds.Genetic manipulation:impact on man and society.Cambridge:Cambridge University Press,1984:161-170.
[6]	董承光, 王娟, 周小凤, 马晓梅, 李生秀, 余渝, 李保成. 基于表型性状的陆地棉种质资源遗传多样性分析[J]. 植物遗传资源学报, 2016, 17(3):438-446.doi:10.13430/j.cnki.jpgr.2016.03.006.
	Dong C G, Wang J, Zhou X F, Ma X M, Li S X, Yu Y, Li B C. Evaluation on genetic diversity of cotton germplasm resources(Gossypium hirsutum L.) on morphological characters[J]. Journal of Plant Genetic Resources, 2016, 17(3):438-446.
[7]	尹会会, 李秋芝, 李海涛, 王士红, 李彤, 商娜, 张晗, 杨中旭. 134份国外陆地棉种质主要农艺性状与纤维品质性状的遗传多样性分析[J]. 植物遗传资源学报, 2017, 18(6):1105-1115.doi:10.13430/j.cnki.jpgr.2017.06.012.
	Yin H H, Li Q Z, Li H T, Wang S H, Li T, Shang N, Zhang H, Yang Z X. Analysis of genetic diversity of the main agronomic and fibre quality characters of 134 foreign upland cotton germplasms[J]. Journal of Plant Genetic Resources, 2017, 18(6):1105-1115. doi: 10.13430/j.cnki.jpgr.2017.06.012
[8]	李慧琴, 于娅, 王鹏, 刘记, 胡伟, 鲁丽丽, 秦文强. 270份陆地棉种质资源农艺性状与品质性状的遗传多样性分析[J]. 植物遗传资源学报, 2019, 20(4):903-910.doi:10.13430/j.cnki.jpgr.20181025003.
	Li H Q, Yu Y, Wang P, Liu J, Hu W, Lu L L, Qin W Q. Genetic diversity analysis of the main agronomic and fiber quality characteristics in 270 upland cotton germplasm resources[J]. Journal of Plant Genetic Resources, 2019, 20(4):903-910.
[9]	金宇豪, 阳会兵, 高倩文, 王峰, 周仲华, 马肖, 文双雅, 胡海燕. 陆地棉纤维品质和农艺性状遗传多样性分析及优良材料鉴定[J]. 东北农业大学学报, 2022, 53(2):1-12.doi:10.19720/j.cnki.1005-9369.2022.02.001.
	Jin Y H, Yang H B, Gao Q W, Wang F, Zhou Z H, Ma X, Wen S Y, Hu H Y. Genetic diversity analysis of fiber quality and agronomic traits and identification of superior materials in upland cotton[J]. Journal of Northeast Agricultural University, 2022, 53(2):1-12.
[10]	Zhao Y L, Wang H M, Chen W, Li Y H, Gong H Y, Sang X H, Huo F C, Zeng F C. Genetic diversity and population structure of elite cotton(Gossypium hirsutum L.) germplasm revealed by SSR markers[J]. Plant Systematics and Evolution, 2015, 301(1):327-336.doi:10.1007/s00606-014-1075-z. URL
[11]	Ai X T, Liang Y J, Wang J D, Zheng J Y, Gong Z L, Guo J P, Li X Y, Qu Y Y. Genetic diversity and structure of elite cotton germplasm(Gossypium hirsutum L.) using genome-wide SNP data[J]. Genetica, 2017, 145(4/5):409-416.doi:10.1007/s10709-017-9976-8. URL
[12]	Wang J D, Zhang Z L, Gong Z L, Liang Y J, Ai X T, Sang Z W, Guo J P, Li X Y, Zheng J Y. Analysis of the genetic structure and diversity of upland cotton groups in different planting areas based on SNP markers[J]. Gene, 2022, 809:146042.doi:10.1016/j.gene.2021.146042. URL
[13]	代攀虹, 孙君灵, 何守朴, 王立如, 贾银华, 潘兆娥, 庞保印, 杜雄明, 王谧. 陆地棉核心种质表型性状遗传多样性分析及综合评价[J]. 中国农业科学, 2016, 49(19):3694-3708.doi:10.3864/j.issn.0578-1752.2016.19.003.
	Dai P H, Sun J L, He S P, Wang L R, Jia Y H, Pan Z E, Pang B Y, Du X M, Wang M. Comprehensive evaluation and genetic diversity analysis of phenotypic traits of core collection in upland cotton[J]. Scientia Agricultura Sinica, 2016, 49(19):3694-3708. doi: 10.3864/j.issn.0578-1752.2016.19.003
[14]	钱玉源, 刘祎, 崔淑芳, 王广恩, 张曦, 金卫平, 李俊兰. 基于表型的棉花种质资源遗传多样性分析及核心种质的抽提[J]. 华北农学报, 2019, 34(S1):29-35.doi:10.7668/hbnxb.20190960.
	Qian Y Y, Liu Y, Cui S F, Wang G E, Zhang X, Jin W P, Li J L. Analysis of genetic diversity of cotton germplasm resources and extraction of core germplasm based on phenotypic traits[J]. Acta Agriculturae Boreali-Sinica, 2019, 34(S1):29-35. doi: 10.7668/hbnxb.20190960
[15]	Han P, Tian X M, Wang Y, Huang C, Ma Y Z, Zhou X F, Yu Y, Zhang D W, Xu H J, Cao Y, Zhu B, Feng Z X, He S P, Du X M, Lin Z X, Zhu L F, You C Y, Pan Z Y, Nie X H. Construction of a core germplasm bank of upland cotton(Gossypium hirsutum L.) based on phenotype,genotype and favorable alleles[J]. Genetic Resources and Crop Evolution, 2022, 69(7):2399-2411.doi:10.1007/s10722-022-01379-6.
[16]	Hu J, Zhu J, Xu H M. Methods of constructing core collections by stepwise clustering with three sampling strategies based on the genotypic values of crops[J]. Theoretical and Applied Genetics, 2000, 101(1/2):264-268.doi:10.1007/s001220051478. URL
[17]	汪磊, 王姣梅, 汪魏, 王玲, 王力军, 严兴初, 谭美莲. 基于表型多样性构建向日葵核心种质[J]. 中国油料作物学报, 2021, 43(6):1052-1060.doi:10.19802/j.issn.1007-9084.2020255.
	Wang L, Wang J M, Wang W, Wang L, Wang L J, Yan X C, Tan M L. Development of a core collection in sunflower(Helianthus annuus L.) germplasm using phenotypic diversity[J]. Chinese Journal of Oil Crop Sciences, 2021, 43(6):1052-1060.
[18]	黄滋康. 中国棉花品种及其系谱[M].修订本. 北京: 中国农业出版社, 2007:252-260.
	Huang Z K. Cotton varieties and their genealogy in China[M]. Revised and enlarged edition. Beijing: China Agriculture Press, 2007:252-260.
[19]	陈光, 杜雄明. 我国陆地棉基础种质表型性状的遗传多样性分析[J]. 西北植物学报, 2006, 26(8):1649-1656.doi:10.3321/j.issn:1000-4025.2006.08.023.
	Chen G, Du X M. Genetic diversity of basal germplasm phenotypes in upland cotton in China[J]. Acta Botanica Boreali-Occidentalia Sinica, 2006, 26(8):1649-1656.
[20]	王秀秀, 邢爱双, 杨茹, 何守朴, 贾银华, 潘兆娥, 王立如, 杜雄明, 宋宪亮. 陆地棉种质资源表型性状综合评价[J]. 中国农业科学, 2022, 55(6):1082-1094.doi:10.3864/j.issn.0578-1752.2022.06.003.
	Wang X X, Xing A S, Yang R, He S P, Jia Y H, Pan Z E, Wang L R, Du X M, Song X L. Comprehensive evaluation of phenotypic characters of nature population in upland cotton[J]. Scientia Agricultura Sinica, 2022, 55(6):1082-1094. doi: 10.3864/j.issn.0578-1752.2022.06.003
[21]	徐云碧, 杨泉女, 郑洪建, 许彦芬, 桑志勤, 郭子锋, 彭海, 张丛, 蓝昊发, 王蕴波, 吴坤生, 陶家军, 张嘉楠. 靶向测序基因型检测(GBTS)技术及其应用[J]. 中国农业科学, 2020, 53(15):2983-3004.doi:10.3864/j.issn.0578-1752.2020.15.001
	Xu Y B, Yang Q N, Zheng H J, Xu Y F, Sang Z Q, Guo Z F, Peng H, Zhang C, Lan H F, Wang Y B, Wu K S, Tao J J, Zhang J N. Genotyping by target sequencing(GBTS)and its applications[J]. Scientia Agricultura Sinica, 2020, 53(15):2983-3004.
[22]	Si Z F, Jin S K, Li J Y, Han Z G, Li Y Q, Wu X N, Ge Y X, Fang L, Zhang T Z, Hu Y. The design,validation,and utility of the "ZJU CottonSNP40K" liquid chip through genotyping by target sequencing[J]. Industrial Crops and Products, 2022, 188:115629.doi:10.1016/j.indcrop.2022.115629. URL

来源地 Source	份数 Number of germplasm resources
来源地 Source	基因型检测 Genotyping	表型调查 Traits investigation
国外Abroad	28	27
黄河流域棉区YRR	264	257
长江流域棉区YtRR	34	33
西北内陆棉区NIR	29	25
北部(特早熟)棉区NSEMR	10	9
华南棉区SCR	2	2
总计Total	367	353

来源地 Source	份数 Number of germplasm resources
来源地 Source	基因型检测 Genotyping	表型调查 Traits investigation
国外Abroad	28	27
黄河流域棉区YRR	264	257
长江流域棉区YtRR	34	33
西北内陆棉区NIR	29	25
北部(特早熟)棉区NSEMR	10	9
华南棉区SCR	2	2
总计Total	367	353

性状 Traits	平均值 Mean	最大值 Max.	最小值 Min.	极差 Range	标准差 s	变异系数/% CV	遗传多样性指数 H'
株高/cm PH	96.86	162.38	68.87	93.51	9.84	10.16	1.99
果枝节位FFBN	6.85	8.83	4.42	4.42	0.62	8.99	1.98
第一果枝高度/cm FFBH	26.10	37.70	14.92	22.78	3.02	11.57	2.04
果枝数FBN	12.52	18.58	9.83	8.75	0.96	7.68	2.00
单株铃数BN	14.11	21.13	7.58	13.55	2.73	19.35	2.10
铃质量/g BW	5.87	7.07	4.15	2.92	0.52	8.82	2.09
衣分/% LP	38.55	44.78	28.76	16.02	2.77	7.18	2.05
纤维长度/mm FL	28.68	34.11	21.93	12.18	1.44	5.02	2.03
断裂比强度/(cN/tex) FS	30.16	42.02	22.95	19.07	2.91	9.65	2.02
马克隆值FM	4.88	6.07	3.43	2.64	0.45	9.18	2.08
皮棉产量/(kg/hm²) LY	995.78	2 180.28	260.28	1 920.0	346.38	34.79	2.03

性状 Traits	平均值 Mean	最大值 Max.	最小值 Min.	极差 Range	标准差 s	变异系数/% CV	遗传多样性指数 H'
株高/cm PH	96.86	162.38	68.87	93.51	9.84	10.16	1.99
果枝节位FFBN	6.85	8.83	4.42	4.42	0.62	8.99	1.98
第一果枝高度/cm FFBH	26.10	37.70	14.92	22.78	3.02	11.57	2.04
果枝数FBN	12.52	18.58	9.83	8.75	0.96	7.68	2.00
单株铃数BN	14.11	21.13	7.58	13.55	2.73	19.35	2.10
铃质量/g BW	5.87	7.07	4.15	2.92	0.52	8.82	2.09
衣分/% LP	38.55	44.78	28.76	16.02	2.77	7.18	2.05
纤维长度/mm FL	28.68	34.11	21.93	12.18	1.44	5.02	2.03
断裂比强度/(cN/tex) FS	30.16	42.02	22.95	19.07	2.91	9.65	2.02
马克隆值FM	4.88	6.07	3.43	2.64	0.45	9.18	2.08
皮棉产量/(kg/hm²) LY	995.78	2 180.28	260.28	1 920.0	346.38	34.79	2.03

类群 Group	Ⅰ群 Group Ⅰ	Ⅱ群 Group Ⅱ	Ⅲ群 Group Ⅲ
性状平均值 Average performance of traits
株高/cm PH	91.04	93.68	103.36
果枝节位FFBN	6.73	6.77	7.00
第一果枝高度/cm FFBH	25.75	25.61	26.87
果枝数FBN	11.74	12.27	13.16
单株铃数BN	11.12	13.45	16.16
铃质量/g BW	5.60	5.74	6.16
衣分/% LP	35.63	37.84	40.66
纤维长度/mm FL	28.51	28.72	28.68
断裂比强度/(cN/tex) FS	29.90	30.17	30.25
马克隆值FM	4.60	4.71	5.22
皮棉产量/(kg/hm²) LY	498.61	852.90	1 382.58
来源地Source		份数No.
国外Abroad	8	18	1
黄河流域棉区YRR	27	111	119
长江流域棉区YtRR	8	23	2
西北内陆棉区NIR	6	13	6
北部(特早熟)棉区NSEMR	3	5	1
华南棉区SCR	0	1	1
总计Total	52	171	130

Please choose a citation manager

Content to export