Abstract:

In order to cultivate a specific type of high-quality wheat,it is necessary to make the quality measurement from the early generation. Due to the limitation of the seed amount in the early generation,only some small measurement methods can be used. Among the commonly used small quantity of quality measurement methods,glutenin swelling index (SIG)and lactate SDS solvent retention capacity (LA-SDS SRC)are better methods to predict wheat quality. The purpose of this study was to compare these two methods and make necessary optimizations,so as to provide support for improving quality breeding efficiency. This study used 8 strong gluten,medium-strong gluten,and medium gluten wheat varieties cultivated in the Huanghuai wheat region as experimental materials. The SIG and LA-SDS SRC values of different materials were measured using whole wheat flour,and the experimental results were compared. The LA-SDS SRC method was further optimized. The results showed that the results measured by SIG and LA-SDS SRC methods were consistent with the order of gluten strength of these varieties. In comparison,LA-SDS SRC method had a better prediction effect on gluten strength and could better reflect the differences between strong gluten varieties and medium gluten varieties;the optimized LA-SDS SRC method (using a 2 mL centrifuge tube)showed a high correlation with the standard LA-SDS SRC method (using a 50 mL centrifuge tube),with a correlation coefficient of up to 0.986. Its predictive effect on gluten strength was superior to the SIG method using a 2 mL centrifuge tube. The standard LA-SDS SRC method and a smaller amount of LA-SDS SRC method are suitable as early generation selection methods for wheat quality breeding in China.

Key words: Wheat, Processing quality, Micro-determination method, SIG, LA-SDS SRC