Effects of Applying Nitrogen Fertilizer on Leaf Senescence Characteristics of Oat in Sandy Land during Grain Filling Stage

doi:10.7668/hbnxb.201750623

Abstract

Abstract: To explore the effect of nitrogen on senescence characteristics of fodder oat leaves,in Horqin sandy land, using oat as material,pure nitrogen of 0 (CK), 70, 140, 210, 280 kg/ha was applied to oats at seedling stage, tillering stage, jointing stage and heading stage in proportion of 15%, 40%, 25% and 20%.Leaf area, chlorophyll content, soluble protein content, antioxidant enzyme activity and membrane peroxidation degree were measured. The results showed that the application of nitrogen fertilizer had an obvious effect on delaying leaf senescence and improving functional leaf time and efficiency.In a certain range with the increased of nitrogen, chlorophyll content, leaf area of oat were significantly improved, the soluble protein content increased, while MDA content decreased, SOD activity decreased significantly, POD activity and CAT activity were significantly enhanced, delaying leaf senescence.When the nitrogen was more than 210 kg/ha, continuing to increase nitrogen application, flag leaf area and chlorophyll content and soluble protein content decreased significantly, and could not improve leaf POD and CAT activity after flowering, but the activity of SOD was significantly increased;MDA content increased significantly.This experiment also indicated that scavenging oxygen free radicals in plants was not a single enzyme action. It depended on the synergistic action of SOD, POD and CAT protective enzymes. The application of 210 kg/ha nitrogen fertilizer in the Horqin sandy land had the strongest anti-aging ability.

Key words: Feeding oat, Nitrogen fertilizer, Leaf, Senescence characteristics

CLC Number:

ZHANG Yuxia, ZHU Aimin, GUO Yuan, DU Xiaoyan, LUAN Ruitao. Effects of Applying Nitrogen Fertilizer on Leaf Senescence Characteristics of Oat in Sandy Land during Grain Filling Stage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2019, 34(1): 124-130. doi: 10.7668/hbnxb.201750623.

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