Study on Binding Pattern of Antennal Binding Protein PxylABP in Plutella xylostella

doi:10.7668/hbnxb.2016.05.036

Abstract

Abstract: Antennal binding proteins are soluble acidic proteins in insect antenna.It is an important way to clarify ABP physiological function by analysising the binding pattern.The PxylABP gene of Plutella xylostella was cloned using reverse transcription PCR and expressed in Escherichia coli.The specificity of PxylABP binding with 29 compounds was tested through fluorescence competitive binding assay,and the molecular docking of PxylABP with compounds were carried out using AutoDock 4.2.The results showed that PxylABP protein had binding ability to Allylisothiocyanate,Hexane,Hexanal,Indole,2-hexanone and β-Lonone,with the dissociation constants of 0.91,1.81,1.17,2.71,2.74 and 14.95 μmol/L,respcetively;The molecular docking results showed that one hydrogen bond generated between Tyr100 and Allyl-isothiocyanate.These data suggested that PxylABP might be involved in the physiological process that discriminate the cruciferous vegetables and herbivore-induced plant volatiles in Plutella xylostella.

Key words: Plutella xylostella, Antennal binding proteins, Fluorescence competitive binding assay, Molecular docking

CLC Number:

DENG Peiyuan, YUAN Wei, LI Yuhua, WANG Linqing, LI Changkan. Study on Binding Pattern of Antennal Binding Protein PxylABP in Plutella xylostella[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(5): 233-238. doi: 10.7668/hbnxb.2016.05.036.

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References

[1] Furlong M J,Wright D J,Dosdall L M.Diamondback moth ecology and management;problems,progress and prospects[J].Annual Review of Entomology,2013,58(1):517-543.
[2] 唐振华.我国昆虫抗药性研究的现状及展望[J].昆虫知识,2000,37(2):97-103.
[3] Pelosi P,Maida R.Odorant-binding proteins in insects[J].Comp Biochem Physiol,1995,111(3):503-514.
[4] Cilek J E,lkediobi C O,llallmon C F,et al.Semi-field evaluation of several novel alkenol analogs of 1-octen-3-ol as attractants to adult Aedes albopictus and Culex Quinquefasciatus[J].Jounral of the American Mosquito Control Association,2011,27(3):256-262.
[5] Hoddle M S,Millar J G,Hoddle C D,et al.Field optimization of the sex pheromone of Sterwma caterrifer (Lepidoptera:Elachistidae):evaluation of lure types,trap height,male flight distances,and number of traps needed per avocado orchard for detection[J].Bulletin of Entomological Research,2011,101(2):145-152.
[6] Ko H J,Park T.Enhancement of odorant detection sensitity by the expresspn of odorant-binding protein[J].Biosens Bioelectron,2008,23(23):1017-1023.
[7] Krieger J,Von Nickisch-Rosenegk E,Mameli M,et al.Binding proteins from the antennae of Bombyx mori[J]. Insect Biochemistry and Molecular Biology,1996,26(3):297-307.
[8] 张婷,刘乃勇,董双林.甜菜夜蛾触角结合蛋白П的cDNA克隆、组织分布及配体结合特性分析[J].昆虫学报,2012,55(5):499-509.
[9] Vogei H,Heidel A J,Heckel D G,et al.Transcriptome analysis of the sex gland of the noctuid moth Heliothis virescens[J].BMC Genomics,2010,11(1):29-49.
[10] Zhang T T,Gu S H,Wu K M,et al.Construction and analysis of cDNA libraries from the antennae of male and female cotton bollworms Helicauerpa armigera (Hübner) and expression analysis of putative odorant-binding protein genes[J].Biochemical and Biophysical Research Commurrications,2011,407(2):393-399.
[11] Li S,Picimbon J F,Ji S D,et al.Multiple functions of an odorant binding protein in the mosquito Aedes aegypti[J].Biochemical and Biophysical Research Communications,2008,372(3):464-468.
[12] Qiao H L,Deng P Y,Li D D,et al.Expression analysis and binding experiments of chemosensory proteins indicate multiple roles in Bombyx mori[J].Journal of Insect Physiology,2013,59(7):667-675.
[13] 王香萍,方宇凌,张钟宁.小菜蛾对合成植物挥发物的活性反应[J].昆虫学报,2005,48(4):503-508.
[14] 祝传书,赵惠燕.虫害诱导的植物挥发物[J].西北农林科技大学学报:自然科学版,2003,31(S1):183-186.
[15] Morris G M,Huey R,Lindstrom W,et al.AutoDock4 and AutoDock Tools4:automated docking with selective receptor flexibility[J].J Comput Chem, 2009,30(16):2785-2791.
[16] Zhou J J,Zhang G A,Huang W,et al.Revisiting odorant-binding protein LUSH of Drosophila melanogaster:evidence for odour recognition and discrimination[J].FEBS Lett,2004,558(1-3):23-26.
[17] Zhou J J,Robertson G,He X L,et al.Characterisation of Bombyx mori odorant-binding proteins reveals that a general odorant-binding protein discriminates between sex pheromone components[J].J Mol Biol,2009,389(3):529-545.
[18] Gong Z J,Zhou W W,Yu H Z,et al.Cloning,expression and functional analysis of a general odorant-binding protein 2 gene of the rice striped stem borer, Chilo suppressulis (Walker) (Lepidoptera:Pyralidae)[J].Insect Mol Biol,2009,18(3):405-417.
[19] Qiao H L,Tuccori E,He X L,et al.Discrimination of alarm pheromone(E)-farnesene by aphid odorant-binding proteins[J].Insect Biochem Mol Biol,2009,39(5-6):414-419.
[20] Gu S H,Wang W X,Zhang X Y,et al.Functional characterization and immunolocalization of odorant binding protein 1 in the lucerne plant bug, Adelphocoris lineolutus (Goeze)[J].Arch Insect Biochem Physiol, 2011,77(2):81-98.
[21] Reddy G V P,Jarvis B J,Slater G P.Olfactory response of Plutella xylostella natural enemies to host pheromone,larval frass,and green leaf cabbage volatiles[J].J Chem Ecol,2002,28(1):131-143.
[22] Reddy G V P,Guerrero A.Interactinm of insect pheromones and plant seminchemicals[J].Trends in Plant Science,2004,9(5):253-261.
[23] Pivnick K A,Jarvis B J,Slater G P.Identification of olfactory cues used in host-plant finding by diamondback moth. Plutella xylostella (Lepidoptera:Plutellidae)[J].J Chem Ecol,1994,20(7):1407-1427.
[24] Reddy G V P,Guerrero A.Behavioral response of the diamondback moth, Plutella xylostella,to green leaf volatiles of Brassica olercea subsp[J].Captata J Agri Food Chem, 2000,48(12):6025-6029.
[25] Lescop E,Briand L,Pernollet J C,et al.Structural basis of the broad specificity of a general odorant-binding protein from honeybee[J].Biochemistry,2009,48(11):2431-2441.
[26] 王海燕,曾艳丽,孟令鹏,等.有关氢键理论研究的现状及前景[J].河北师范大学学报:自然科学版,2005,29(2):177-181.

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