Inheritance and Gene Mapping of Mosaic-leaf in Brassica napus L.

doi:10.7668/hbnxb.2016.04.007

Abstract

Abstract: In order to study the genetic characteristic of mosaic-leaf and identify the molecular markers linked to mosaic-leaf in Brassica napus L..In this study,three segregated populations,F₁,BC₁ and F₂,were constructed by crossing two parent lines,2205 (round-leaf) and 1423 (mosaic-leaf).The mosaic-leaf line of two parents and the segregated populations were used to analyze the inheritance of mosaic-leaf;molecular marker technology was used to map the mosaic-leaf gene.The results showed that the leaf morphology of F₁ plants revealed mosaic-leaf phenotype and the separated proportion of mosaic-leaf to round leaf in BC₁ (F₁×2205) and F₂ was fitted to 1:1 and 3:1,respectively,which showed that the mosaic leaf was controlled by a pair of incomplete dominant gene.Meanwhile,637 pairs of SSR primers combined with bulked segregation analysis (BSA) were employed to identify the markers linked to mosaic-leaf gene.As a result,three markers:CB10079,BNGMS114 and BNGMS385,linked to the mosaic-leaf gene were identified.All of them were located in one side of the gene,and BNGMS114 was closest to the gene with a distance of 2.5 cM.The sequences of three markers were submitted to blast with those of B.rapa genome.The results indicated that the sequences of three markers showed good collinearity with those of A10 chromosome.The target gene was located in the downstream section of 15.70 Mb on A10.These results will be useful for MAS and the mosaic-leaf gene cloning.

Key words: Brassica napus L., Mosaic-leaf, Genetics, SSR

CLC Number:

S635.03
Q78

LANG Lina, ZHAO Na, MA Bing, LIU Yaping, TIAN Zhengshu, LIU Lu, LU Hong, LIU Xia, WANG Yang, XU Aixia, HUANG Zhen. Inheritance and Gene Mapping of Mosaic-leaf in Brassica napus L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2016, 31(4): 39-43. doi: 10.7668/hbnxb.2016.04.007.

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