Abstract: To study the characteristics of the aminopeptidase gene in white-tip nematode,and to provide theory support for the prevention.According to the transcriptome resequencing,an aminopeptidase gene of A.besseyi was isolated by RACE method,and designed as Ab-Ampep.Bioinformatic and in-situ hybridization were used for the gene analysis.The coding sequence was 2 787 bp including a 2 661 bp open reading frame (ORF),which encoded an 886 amino acid protein.The putative protein molecular weight was 101.74 kDa and its theoretical isoelectric point was 5.78.The deduced amino acid sequence contains two conserved zinc-binding sites and a PepN Multi-domain.It belongs to GluZincin Superfamily,which contained the characteristic conserved sequence of HE xx H.The multiple sequence alignment showed that the functional region was conserved,and the highest level of similarity was 50% to a aminopeptidase(ERG83308.1)from Ascaris suum in GenBank.The phylogenetic tree constructed on the basis of amino acid sequences clearly showed that the relationship of Ab-Ampep was most intimate with aminopeptidase(ERG83308.1)from A.suum. Protein structure analysis indicated that the main structural element in Ab-Ampep protein was α-Helix.In situ hybridization indicated that enhanced hybridization signals were observed in genital area,and the expression of Ab-Ampep was similarly in genital area of A.besseyi.Though the functional analysis of aminopeptidase gene,molecular technology or enzyme inhibitor was used to inhibit the expression of aminopeptidase.It may affect the nematode development and provide a new way for prevention.

Key words: Aphelenchoides besseyi, Aminopeptidase gene, Gene cloning, Bioinformatics analysis, In situ hybridization

CLC Number: 

• S435.111.48

• Q786