Sequence Analysis and Expression Level Detection of MITF-M Gene in Different Color Skin Tissues of Yak

doi:10.7668/hbnxb.2015.03.001

Abstract

Abstract: The aim of this present study are the investigation of the MITF-M gene coding region sequences,the prediction of its translated protein structures and the dictation of its transcription mRNA expression level in skin tissues of yaks with black and white skin color.This study was aim to reveal of the molecular mechanism of skin color formation in yak.In this study,a coding region sequence of yak MITF-M was cloned by RT-PCR technique.Characters of the MITF-M gene encoded protein sequences were predicted and analyzed by the methods of bioinformatics in basically chemical properties and secondary structure.The expression levels of MITF-M mRNA in skin tissues of yak were detected by semi-quantitative PCR.Quantitative PCR was employed to examine the expression levels of MITF-M mRNA in different color yak skin tissues.As results,MITF-M gene was cloned,which contains a complete ORF of 1 242 bp.This ORF encoding a peptides chain which contain 413 amino acids.The second structure of this peptides chain was contain helix,coli and extended strand.And the expression level of MITF-M mRNA in skin tissues between dark yak and white yak were extremely remarkable (P<0.01).

Key words: Yak, MITF-M gene, Cloning, Bioinformatic analysis, Expression level

CLC Number:

ZHANG Jian-yi, LIANG Chun-nian, PEI Jie, GUO Xian, SHEN You-ming, YAN Ping. Sequence Analysis and Expression Level Detection of MITF-M Gene in Different Color Skin Tissues of Yak[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2015, 30(3): 1-7. doi: 10.7668/hbnxb.2015.03.001.

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